Metabolomic and elemental profiling of human tissue in kidney cancer.
Metabolomic and elemental profiling of human tissue in kidney cancer.
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DOI：
10.1007/s11306-021-01779-2
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发表时间：
2021-03-04
期刊：
影响因子：
--
通讯作者：
Ruman T
中科院分区：
文献类型：
--
作者：
Nizioł J;Copié V;Tripet BP;Nogueira LB;Nogueira KOPC;Ossoliński K;Arendowski A;Ruman T
肾癌是最常诊断和最致命的泌尿系癌症之一。尽管治疗取得了进展，但目前还没有特定的生物标志物用于指导治疗干预。这项工作的主要目的是对人类肾癌和正常组织进行代谢组学和元素分析，并评估癌症生物标志物。使用三种不同的分析方法对 50 名肾癌患者的肿瘤和邻近正常人肾组织进行代谢和元素分析。使用高分辨率核磁共振波谱鉴定并量化了肾癌的五种潜在组织生物标志物。使用电感耦合等离子体发射光谱法分析组织中选定化学元素的含量。使用银 109 纳米粒子增强钢靶激光解吸/电离质谱法检测了区分肾癌和正常组织的 11 种质谱特征。我们的结果源自 ICP-OES、LDI MS 和 1 H NMR 方法的组合，表明本文鉴定的组织生物标志物似乎在肾癌的临床预后和/或诊断中具有巨大的潜力。在线版本包含可在 10.1007/s11306-021-01779-2 获取的补充材料。
Kidney cancer is one of the most frequently diagnosed and the most lethal urinary cancer. Despite advances in treatment, no specific biomarker is currently in use to guide therapeutic interventions. Major aim of this work was to perform metabolomic and elemental profiling of human kidney cancer and normal tissue and to evaluate cancer biomarkers. Metabolic and elemental profiling of tumor and adjacent normal human kidney tissue from 50 patients with kidney cancer was undertaken using three different analytical methods. Five potential tissue biomarkers of kidney cancer were identified and quantified using with high-resolution nuclear magnetic resonance spectroscopy. The contents of selected chemical elements in tissues was analyzed using inductively coupled plasma optical emission spectrometry. Eleven mass spectral features differentiating between kidney cancer and normal tissues were detected using silver-109 nanoparticle enhanced steel target laser desorption/ionization mass spectrometry. Our results, derived from the combination of ICP-OES, LDI MS and 1H NMR methods, suggest that tissue biomarkers identified herein appeared to have great potential for use in clinical prognosis and/or diagnosis of kidney cancer. The online version contains supplementary material available at 10.1007/s11306-021-01779-2.
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影响因子：
--
作者：
Lucarelli G;Galleggiante V;Rutigliano M;Sanguedolce F;Cagiano S;Bufo P;Lastilla G;Maiorano E;Ribatti D;Giglio A;Serino G;Vavallo A;Bettocchi C;Selvaggi FP;Battaglia M;Ditonno P
通讯作者：
Ditonno P
影响因子：
23.4
作者：
Dobrowolski, Z;Drewniak, T;Jakubik, P
通讯作者：
Jakubik, P
通过FDG PET/CT评估的肾细胞癌中葡萄糖积累的加速表明，抗酪氨酸激酶抑制剂治疗的耐药性。
影响因子：
3.8
作者：
Nakaigawa N;Kondo K;Ueno D;Namura K;Makiyama K;Kobayashi K;Shioi K;Ikeda I;Kishida T;Kaneta T;Minamimoto R;Tateishi U;Inoue T;Yao M
通讯作者：
Yao M
影响因子：
2.5
作者：
Kim HS;Kim YJ;Seo YR
通讯作者：
Seo YR
影响因子：
5.8
作者：
Abdel-Gawad, Mahmoud;Elsobky, Emad;Ali-El-Dein, Bedeir
通讯作者：
Ali-El-Dein, Bedeir