Skeletal myogenesis on elastomeric substrates: implications for tissue engineering.
Skeletal myogenesis on elastomeric substrates: implications for tissue engineering.
复制标题
弹性体基质上的骨骼肌生成：对组织工程的影响。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
P. Tresco
中科院分区：
文献类型：
--
作者：
M. M. Mulder;R. Hitchcock;P. Tresco
旨在了解骨骼肌和生物材料之间的相互作用的研究可能会为各种新兴技术的发展提供有用的信息，从转基因细胞的新型运载工具到功能齐全的骨骼肌组织。为了确定弹性体材料作为此类应用的基底的实用性，我们询问骨骼肌发生是否会支持市售聚氨酯Tecoflex SG-80 A。G8骨骼肌成肌细胞培养Tecoflex二维固体薄膜通过旋转铸造方法制造。成肌细胞附着、增殖、显示迁移活性并分化成多核肌管，其在固体薄膜上表达肌球蛋白重链，表明Tecoflex SG-80 A允许骨骼肌发生。多孔三维（3-D）的细胞支架制成的各种形状，厚度和孔隙率的浸渍沉淀法，并随后与显微镜和机械的方法进行了表征。机械分析显示，结构是弹性的，在100%伸长后恢复其原始长度。用肌肉前体接种3-D基质以确定是否可以在制造的构建物的多孔网络内获得肌肉分化。培养数周后，组织学研究显示弹性材料内存在多核肌管。此外，免疫组织化学分析表明，肌管表达肌球蛋白重链蛋白，表明肌管已达到终末分化状态。总之，研究结果表明，它确实是可行的工程生物人工系统组成的骨骼肌培养在三维弹性基板。
Studies geared towards understanding the interaction between skeletal muscle and biomaterials may provide useful information for the development of various emerging technologies, ranging from novel delivery vehicles for genetically modified cells to fully functional skeletal muscle tissue. To determine the utility of elastomeric materials as substrates for such applications, we asked whether skeletal myogenesis would be supported on a commercially available polyurethane, Tecoflex SG-80A. G8 skeletal myoblasts were cultured on Tecoflex two-dimensional solid thin films fabricated by a spin-casting method. Myoblasts attached, proliferated, displayed migratory activity and differentiated into multinucleated myotubes which expressed myosin heavy chain on solid thin films indicating that Tecoflex SG-80A was permissive for skeletal myogenesis. Porous three-dimensional (3-D) cell scaffolds were fabricated in a variety of shapes, thicknesses, and porosities by an immersion precipitation method, and where subsequently characterized with microscopic and mechanical methods. Mechanical analysis revealed that the constructs were elastomeric, recovering their original length following 100% elongation. The 3-D substrates were seeded with muscle precursors to determine if muscle differentiation could be obtained within the porous network of the fabricated constructs. Following several weeks in culture, histological studies revealed the presence of multinucleated myotubes within the elastomeric material. In addition, immunohistochemical analysis indicated that the myotubes expressed the myosin heavy chain protein suggesting that the myotubes had reached a state of terminal differentiation. Together the results of the study suggest that it is indeed feasible to engineer bioartificial systems consisting of skeletal muscle cultivated on a 3-D elastomeric substrate.
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抑肽酶是一种库尼茨型蛋白酶抑制剂，可刺激骨骼肌分化。
DOI：
10.1242/dev.120.12.3639
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Wells,JM;Strickland,S
通讯作者：
Strickland,S
影响因子：
56.9
作者：
BARR, E;LEIDEN, JM
通讯作者：
LEIDEN, JM
影响因子：
4
作者：
S. Swasdison;Richard Mayne
通讯作者：
S. Swasdison;Richard Mayne
影响因子：
56.9
作者：
DHAWAN, J;PAN, LC;BLAU, HM
通讯作者：
BLAU, HM
DOI：
10.1002/jbm.820270104
发表时间：
1993-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
FREED, LE;MARQUIS, JC;LANGER, R
通讯作者：
LANGER, R