Long-Distance Transport of Prosystemin Messenger RNA in Tomato

Long-Distance Transport of Prosystemin Messenger RNA in Tomato
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番茄中前系统蛋白信使 RNA 的长距离运输

DOI:
10.3389/fpls.2017.01894
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发表时间:
2017-11
影响因子:
5.6
通讯作者:
Hu Yuanyuan
Hu Yuanyuan
中科院分区:
生物学2区
文献类型:
--
作者:
Zhang Haiyan;Hu Yuanyuan

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主要结论:转基因前系统蛋白(PS)基因的转录本是可移动的,并且PS mRNA在番茄和烟草植物中可以翻译成蛋白质。系统素(SYS)及其前体蛋白前系统素(PS)是番茄伤口诱导信号通路的上游成分。尽管伤口反应的移动信号已成为大量研究的主题,但其身份仍然存在争议。深入研究揭示了 mRNA 对植物系统信号传导的重要作用。我们假设 PS mRNA 可以作为番茄中的可传递信号。在此,我们通过瞬时农杆菌渗透测定系统证明转基因 PS mRNA 发生在距离其产生初始位点相当远的叶子中。我们还表明 PS 蛋白存在于远处叶子的维管束中。我们的结果表明,转基因 PS mRNA 可能作为长距离信号发挥作用,调节番茄的系统防御反应,为 SYS 信号传输的多方面系统提供新的见解。
Main conclusion: The transcripts of transgenic prosystemin (PS) gene are mobile and the PS mRNA can be translated into protein in tomato and tobacco plants. Systemin (SYS) and its precursor protein, prosystemin (PS), are upstream components of the wound-induced signaling pathway in tomato. Although the mobile signal(s) for wound responses has been the subject of considerable research, its identity remains controversial. Intensive studies have revealed the essential role of mRNA on plant systemic signaling. We hypothesize that PS mRNA can act as a transmissible signal in tomato. Herein, we demonstrated that transgenic PS mRNA occurs in leaves located at considerable distances from the initial site of its generation by a transient Agrobacterium-infiltration assay system. We also showed that PS protein is present in the vascular bundle of the distant leaves. Our results indicate that transgenic PS mRNA may be functional as a long-distance signal to modulate systemic defense responses in tomato, providing novel insights into the multifaceted systems by which SYS signaling transports.
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