Immunodominance hierarchy after seasonal influenza vaccination.
Immunodominance hierarchy after seasonal influenza vaccination.
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DOI：
10.1080/22221751.2022.2135460
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发表时间：
2022-12
影响因子：
13.2
通讯作者：
中科院分区：
文献类型：
--
作者：
目前的流感疫苗引起针对流感病毒的血凝素（HA）蛋白的体液免疫应答。在HA头部中已鉴定出不同的抗原位点作为血凝抑制（HAI）抗体（Sb、Sa、Cb、Ca 1和Ca 2）的主要靶标。为了确定每个位点的免疫显性（ID），我们对一组突变病毒进行了HAI测定，每种突变病毒都缺乏一个经典定义的抗原位点，并将其与野生型（Wt）进行比较。在两种不同方案的疫苗接种前后测量凝集抗体：年轻人接种四价流感疫苗（QIV）;老年人接种三价流感疫苗（ATIV）。我们的结果显示，与Wt相比，老年人接种疫苗前针对所有抗原位点的绝对值显著降低，而年轻人仅针对Sb和Ca 2。与ATIV的Wt相比，对所有病毒的疫苗接种的体液应答均显著降低，而对于QIV，仅对Sb和Ca 2的体液应答显著降低。在所有情况下，观察到最强的减少锑其次是钙。我们得出结论，ID配置文件是明确占主导地位的Sb其次是钙。此外，抗体反应随着年龄的增长而演变，增加了对HA头部免疫显性表位较少的反应。佐剂可以积极影响对HA头部的多个抗原位点的ID层次拓宽反应。
Current influenza vaccines elicit humoral immune responses against the haemagglutinin (HA) protein of influenza viruses. Different antigenic sites have been identified in the HA head as the main target of haemagglutination inhibition (HAI) antibodies (Sb, Sa, Cb, Ca1 and Ca2). To determine immunodominance (ID) of each site, we performed HAI assays against a panel of mutant viruses, each one lacking one of the classically defined antigenic sites and compared it to wild type (Wt). Agglutinating antibodies were measured before and after vaccination in two different regimens: Quadrivalent Influenza Vaccine (QIV) in young adults; or Adjuvanted Trivalent influenza Vaccine (ATIV) in elderly. Our results showed abs before vaccination were significantly reduced against all antigenic sites in the elderly and only against Sb and Ca2 in young adults compared to the Wt. Humoral response to vaccination was significantly reduced against all viruses compared to the Wt for the ATIV and only against Sb and Ca2 for the QIV. The strongest reduction was observed in all cases against Sb followed by Ca2. We concluded that ID profile was clearly dominated by Sb followed by Ca2. Additionally, the antibody response evolved with age, increasing the response towards less immunodominant epitopes of HA head. Adjuvants can positively influence ID hierarchy broadening responses towards multiple antigenic sites of HA head.
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DOI：
10.1073/pnas.0903181106
发表时间：
2009-05-12
影响因子：
11.1
作者：
Galli, Grazia;Hancock, Kathy;Stephenson, Iain
通讯作者：
Stephenson, Iain
DOI：
10.1038/s41572-018-0002-y
发表时间：
2018-06-28
期刊：
Nature reviews. Disease primers
影响因子：
--
作者：
Krammer F;Smith GJD;Fouchier RAM;Peiris M;Kedzierska K;Doherty PC;Palese P;Shaw ML;Treanor J;Webster RG;García-Sastre A
通讯作者：
García-Sastre A
影响因子：
4.6
作者：
Kirkpatrick E;Qiu X;Wilson PC;Bahl J;Krammer F
通讯作者：
Krammer F
影响因子：
2.2
作者：
Altman, Meghan O.;Angeletti, Davide;Yewdell, Jonathan W.
通讯作者：
Yewdell, Jonathan W.
影响因子：
64.5
作者：
CATON, AJ;BROWNLEE, GG;GERHARD, W
通讯作者：
GERHARD, W