Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes.
Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes.
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DOI：
10.1016/0168-1702(88)90039-1
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发表时间：
1988-09
期刊：
影响因子：
5
通讯作者：
Mockett AP
中科院分区：
文献类型：
--
作者：
Cavanagh D;Davis PJ;Mockett AP
IBV的刺突糖蛋白（S）基因编码前体蛋白，其被切割成N-末端S1和C-末端S2糖多肽。诱导中和抗体的S1糖多肽包含约520个氨基酸残基。我们已经确定了马萨诸塞州（Mass）血清型的七个菌株的S1的核苷酸序列和另外两个Mass菌株的前337个碱基。尽管这些菌株已经在欧洲和美国分离了三十多年，但在该组内只有4%的碱基和6%的氨基酸变异。S1中32个氨基酸差异的近三分之一位于两个高变区（HVR 1和2），包括残基38-51和99-115，由Niesters等人（1986）鉴定，表明HVR 1和2是Mass血清型的特征。HVR 1和HVR 2内的氨基酸变异分别为29%和40%。通过对前337个核苷酸的测序，可以将五种疫苗株彼此区分开。抵抗两种单克隆抗体（A13和A38）中和的M41变体在134位有相同的单碱基变化，导致脯氨酸残基45被组氨酸取代。这表明HVR 1内的残基与诱导中和抗体的表位相关。
The spike glycoprotein (S) gene of IBV codes for a precursor protein which is cleaved into the N-terminal S1 and C-terminal S2 glycopolypeptides. The S1 glycopolypeptide, which induces neutralizing antibody, comprises approximately 520 amino acid residues. We have determined the nucleotide sequence of S1 of seven strains of the Massachusetts (Mass) serotype and the first 337 bases of two additional Mass strains. Despite the fact that the strains had been isolated over three decades in Europe and the U.S.A. there was only 4% base and 6% amino acid variation within the group. Nearly one third of the 32 amino acid differences in S1 were in two hypervariable regions (HVRs 1 and 2) comprising residues 38–51 and 99–115, identified by Niesters et al. (1986), showing that HVRs 1 and 2 are a feature of the Mass serotype. Amino acid variation within HVRs 1 and 2 was 29% and 40% respectively. Five vaccine strains could be distinguished from each other by sequencing of the first 337 nucleotides. Variants of M41 which resisted neutralization by two monoclonal antibodies (A13 and A38) had the same, single base change at position 134, resulting in substitution of proline residue 45 by histidine. This indicates that residues within HVR 1 are associated with epitopes which induce neutralizing antibody.
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