Extracellular redox potential regulation improves yeast tolerance to furfural
Extracellular redox potential regulation improves yeast tolerance to furfural
复制标题
细胞外氧化还原电位调节提高酵母对糠醛的耐受性
DOI：
10.1016/j.ces.2018.11.059
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发表时间：
2019-03
影响因子：
4.7
通讯作者：
Bai Feng-Wu
中科院分区：
文献类型：
--
作者：
Li Kai;Xia Juan;Mehmood Muhammad Aamer;Zhao Xin-Qing;Liu Chen-Guang;Bai Feng-Wu
糠醛是木质纤维素生物质水解产物中的主要有毒副产物，对酿酒酵母的生长和乙醇发酵有抑制作用。为了增强酵母对糠醛的耐受性，通过调节发酵系统的通气，将胞外氧化还原电位（ORP）分别控制在-150 mV、-100 mV和-50 mV，从而对发酵系统进行细胞外氧化还原电位（ORP）调节。当ORP控制在-100 mV时，酵母细胞表现出更好的生长、糠醛降解和乙醇生产。细胞内氧化还原对如NADH/NAD+和GSH/GSSG的分析表明细胞外ORP和细胞内氧化还原稳态之间的相关性。氧化还原控制条件下，糠醛胁迫引起的细胞内活性氧减少，从而赋予酵母细胞对糠醛的耐受性。对在-100 mV ORP控制下取样的酵母细胞的比较转录组分析进一步揭示了增殖基因的表达被上调以更有效地降解糠醛。这些结果表明，胞外ORP调节将是提高纤维素乙醇发酵过程中酵母对糠醛胁迫耐受性的策略。
Furfural is a major toxic byproduct present in the hydrolysate of lignocellulosic biomass, which inhibits the growth and ethanol fermentation ofSaccharomyces cerevisiae. To enhance yeast tolerance to furfural, extracellular redox potential (ORP) regulation was employed for the fermentation system through controlling ORP at −150 mV, −100 mV and −50 mV, respectively, by adjusting its aeration. When ORP was controlled at −100 mV, yeast cells exhibited improved growth, furfural degradation and ethanol production. Analysis of intracellular redox pairs such as NADH/NAD+and GSH/GSSG indicated a correlation between extracellular ORP and intracellular redox homeostasis. Moreover, intracellular reactive oxygen species (ROS) caused by furfural stress decreased under the redox control condition, which consequently conferred yeast cells tolerance to furfural. Comparative transcriptome analysis for yeast cells sampling under the ORP control at −100 mV further revealed that the expression of genes for proliferation was up-regulated to degrade furfural more efficiently. These results demonstrated that extracellular ORP regulation would be a strategy for enhancing yeast tolerance to furfural stress during cellulosic ethanol fermentation.
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