Morphological adaptation of sheep's rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis.
Morphological adaptation of sheep's rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis.
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绵羊瘤胃上皮对高谷物饮食的形态适应需要改变参与细胞周期调节、细胞增殖和凋亡的基因表达
DOI：
10.1186/s40104-018-0247-z
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发表时间：
2018
影响因子：
7
通讯作者：
Mao S
中科院分区：
文献类型：
--
作者：
Xu L;Wang Y;Liu J;Zhu W;Mao S
本研究的目的是表征高谷物（HG）饮食适应过程中绵羊瘤胃上皮（RE）细胞周期调控、细胞增殖和凋亡相关候选基因和蛋白的相对mRNA表达变化。将20只绵羊分为4组，每组5只动物。这些动物被分配到不同时期的HG饮食（含有40%的饲料和60%的浓缩物混合物）喂养。HG组分别饲喂HG饲料7 d（G7，n = 5）、14 d（G14，n = 5）和28 d（G28，n = 5）。对照组（CON，n = 5）饲喂粗饲料28 d。结果表明，饲喂HG使瘤胃pH呈线性下降（P < 0.001），瘤胃总挥发性脂肪酸（TFA）、丁酸（BFA）、戊酸（VA）浓度呈线性上升（P = 0.001），血浆IGF-1浓度呈二次曲线上升（P = 0.043）。饲喂HG日粮14 d后，瘤胃乳头的长度（平方根，P = 0.004）、宽度（立方根，P = 0.015）和表面（直线根，P = 0.003）均增大。高糖组大鼠皮肤角质层细胞层数增加（P < 0.001），角质层厚度增加（P <0.001），基底层厚度增加（P < 0.001）。RE中基底层细胞比例在G 0/G1期呈直线下降（P < 0.001），在S期呈直线上升（P = 0.006），在G2/M期呈立方上升（P = 0.004）。G7、G14和G28组凋亡细胞比例较CON组降低（二次曲线，P < 0.001）。高糖饲料喂养可线性降低Cyclin E1（P = 0.021）和CDK-2（P = 0.001）的mRNA表达和Cyclin E1的蛋白表达（P = 0.027）。饲喂高糖饲料可使IGFBP-2（P = 0.034）和IGFBP 5（P < 0.009）基因的mRNA表达量线性增加，而IGFBP 3的表达量线性降低（P < 0.001）。细胞凋亡相关基因Caspase 8的表达呈二次曲线下降（P = 0.012），Bad mRNA的表达呈三次曲线下降（P = 0.053）。这些结果表明，随着时间的推移，在瘤胃乳头的大小，细胞周期调控和基因参与的增殖和凋亡的高谷物饲料喂养绵羊的顺序变化。本文的在线版本（10.1186/s40104-018-0247-z）包含补充材料，可供授权用户使用。
The objectives of this study were to characterize changes in the relative mRNA expression of candidate genes and proteins involved in cell cycle regulation, cell proliferation and apoptosis in the ruminal epithelium (RE) of sheep during high-grain (HG) diet adaptation. Twenty sheep were assigned to four groups with five animals each. These animals were assigned to different periods of HG diet (containing 40% forage and 60% concentrate mix) feeding. The HG groups received an HG diet for 7 (G7, n = 5), 14 (G14, n = 5) and 28 d (G28, n = 5), respectively. In contrast, the control group (CON, n = 5) was fed the forage-based diet for 28 d. The results showed that HG feeding linearly decreased (P < 0.001) the ruminal pH, and increased the concentrations of ruminal total volatile fatty acid (linear, P = 0.001), butyrate (linear, P < 0.001), valerate (quadratic P = 0.029) and the level of IGF-1 (quadratic, P = 0.043) in plasma. The length (quadratic, P = 0.004), width (cubic, P = 0.015) and surface of the ruminal papillae (linear, P = 0.003) were all enlarged after 14 d of HG diet feeding. HG feeding cubically increased the number of cell layers forming the stratum corneum (SC, P < 0.001) and the thickness of the SC (P < 0.001) and stratum basale (P < 0.001). The proportion of basal layer cells in the RE decreased (linear, P < 0.001) in the G0/G1-phase, but it increased linearly (P = 0.006) in the S-phase and cubically (P = 0.004) in the G2/M-phases. The proportion of apoptosis cells in G7, G14 and G28 was reduced compared to the CON (quadratic, P < 0.001). HG diet feeding linearly decreased the mRNA expression of Cyclin E1 (P = 0.021) and CDK-2 (P = 0.001) and (P = 0.027) the protein expression of Cyclin E1. Feeding an HG diet linearly increased the mRNA expression of genes IGFBP-2 (P = 0.034) and IGFBP 5 (P < 0.009), while linearly decreasing (P < 0.001) the IGFBP 3 expression. The expression of cell apoptosis gene Caspase 8 decreased (quadratic, P = 0.012), while Bad mRNA expression tended to decrease (cubic, P = 0.053) after HG feeding. These results demonstrated sequential changes in rumen papillae size, cell cycle regulation and the genes involved in proliferation and apoptosis as time elapsed in feeding a high-grain diet to sheep. The online version of this article (10.1186/s40104-018-0247-z) contains supplementary material, which is available to authorized users.
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影响因子：
10.5
作者：
Clurman, BE;Sheaff, RJ;Roberts, JM
通讯作者：
Roberts, JM
影响因子：
56.9
作者：
KOFF, A;GIORDANO, A;ROBERTS, JM
通讯作者：
ROBERTS, JM
影响因子：
2
作者：
Etschmann, Benjamin;Suplie, Annabelle;Martens, Holger
通讯作者：
Martens, Holger
影响因子：
2.4
作者：
GALFI, P;MARTENS, H
通讯作者：
MARTENS, H
影响因子：
0.9
作者：
Gálfi, P;Neogrády, S;Gäbel, G
通讯作者：
Gäbel, G