Telomere length is highly heritable and independent of growth rate manipulated by temperature in field crickets
Telomere length is highly heritable and independent of growth rate manipulated by temperature in field crickets
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田间蟋蟀的端粒长度具有高度遗传性，并且与温度控制的生长速率无关
DOI：
10.1101/2020.05.29.123216
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Boonekamp J
中科院分区：
文献类型：
--
作者：
Boonekamp J
许多生物体能够比它们本身生长得更快。抑制生长率在功能上可以通过加速生长对寿命的负面影响来解释。然而，潜在的机制仍然难以捉摸。端粒磨损被认为是一种致病因素，并且主要在吸热脊椎动物中进行研究。我们使用末端限制性片段和 Bal 31 方法确定端粒以染色体末端的形式存在于模型昆虫田蟋蟀中。端粒包含平均 38 kb 的 TTAGGn 重复序列，比人类婴儿的端粒长四倍多。Bal 31 测定证实端粒重复序列位于染色体末端。我们测试了第 1 天、第 65 天、第 85 天和第 125 天之间的快速生长是否是以牺牲端粒长度为代价实现的，方法是将在 23°C 下饲养的若虫与其在 28°C 下饲养的兄弟姐妹进行比较，后者在最初 65 天的生长速度快了三倍。令人惊讶的是，温度处理和年龄都不影响平均端粒长度。与此同时，端粒长度的广义遗传力非常高，约为 100%。尽管遗传力很高，但进化性（遗传方差的平均标准化测量）相对于体重来说较低。我们在端粒进化的背景下讨论我们的发现。脊椎动物端粒生物学的一些重要特征在可追溯到三叠纪的昆虫物种中很明显。生长速率对端粒长度的明显缺乏影响令人费解，这表明在生长阶段端粒长度的维持能力很强。这种端粒长度的维持是否具有适应性仍然难以捉摸，需要进一步研究与野外适应性的联系。
Many organisms are capable of growing faster than they do. Restrained growth rate has functionally been explained by negative effects on lifespan of accelerated growth. However, the underlying mechanisms remain elusive. Telomere attrition has been proposed as a causal agent and has been mostly studied in endothermic vertebrates. We established that telomeres exist as chromosomal‐ends in a model insect, the field cricketGryllus campestris, using terminal restriction fragment andBal 31methods. Telomeres comprised TTAGGn repeats of 38 kb on average, more than four times longer than the telomeres of human infants.Bal 31assays confirmed that telomeric repeats were located at the chromosome‐ends. We tested whether rapid growth between day 1, day 65, day 85, and day 125 is achieved at the expense of telomere length by comparing nymphs reared at 23°C with their siblings reared at 28°C, which grew three times faster in the initial 65 days. Surprisingly, neither temperature treatment nor age affected average telomere length. Concomitantly, the broad sense heritability of telomere length was remarkably high at ~100%. Despite high heritability, the evolvability (a mean‐standardized measure of genetic variance) was low relative to that of body mass. We discuss our findings in the context of telomere evolution. Some important features of vertebrate telomere biology are evident in an insect species dating back to the Triassic. The apparent lack of an effect of growth rate on telomere length is puzzling, suggesting strong telomere length maintenance during the growth phase. Whether such maintenance of telomere length is adaptive remains elusive and requires further study investigating the links with fitness in the wild.
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影响因子：
4.5
作者：
Bauch, Christina;Boonekamp, Jelle J.;Verhulst, Simon
通讯作者：
Verhulst, Simon
成年豆娘和蜻蜓（蜻蜓目）衰老的比较分析
影响因子：
2.1
作者：
T. Sherratt;Christopher Hassall;Robert A. Laird;David J. Thompson;Adolfo Cordero
通讯作者：
Adolfo Cordero
大西洋鲑鱼早期发育过程中的端粒伸长与环境温度无关
影响因子：
2.8
作者：
Darryl McLennan;J. Armstrong;D. C. Stewart;S. Mckelvey;W. Boner;P. Monaghan;N. Metcalfe
通讯作者：
N. Metcalfe
胚胎发育的速度和稳定性影响端粒动力学：早熟鸟类模型的实验研究
DOI：
10.1098/rspb.2020.1378
发表时间：
2020
期刊：
Proceedings of the Royal Society B
影响因子：
--
作者：
A. Stier;N. Metcalfe;P. Monaghan
通讯作者：
P. Monaghan
端粒长度是可遗传的，并且与野生鸟类的寿命具有遗传相关性
影响因子：
4.9
作者：
Oscar Vedder;M. Moiron;Coraline Bichet;C. Bauch;S. Verhulst;P. Becker;S. Bouwhuis
通讯作者：
S. Bouwhuis