Sm2, a paralog of the Trichoderma cerato-platanin elicitor Sm1, is also highly important for plant protection conferred by the fungal-root interaction of Trichoderma with maize.
Sm2, a paralog of the Trichoderma cerato-platanin elicitor Sm1, is also highly important for plant protection conferred by the fungal-root interaction of Trichoderma with maize.
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DOI：
10.1186/s12866-014-0333-0
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发表时间：
2015-01-16
期刊：
影响因子：
4.2
通讯作者：
Seidl-Seiboth V
中科院分区：
文献类型：
--
作者：
Gaderer R;Lamdan NL;Frischmann A;Sulyok M;Krska R;Horwitz BA;Seidl-Seiboth V
来自生防真菌绿色木霉的蛋白质Sm 1和Sm 2属于角铂蛋白家族。该家族的成员是小的分泌蛋白，其由具有所有类型生活方式的丝状真菌大量产生。木霉属真菌的一些物种被认为是生防真菌，因为它们是真菌寄生虫，也能够直接与植物相互作用，从而刺激植物防御反应。以前的研究表明，来自T.绿色木霉-以及在较小程度上其来自深绿木霉的同源物Epl 1-诱导植物防御反应。然而，木霉属中的cerato-platanin蛋白家族的其他成员的植物保护潜力尚未被研究。为了分析角Platanin蛋白Sm 2的功能，产生并表征了sm 1和sm 2敲除菌株。Sm 1和Sm 2缺失对T.在玉米幼苗中，用植物病原体异盘孢菌（Cochliobolus heterostrophus）挑战，测试了virens对诱导系统抗性的作用。这些植物实验也用T.深绿素EPL 1和EPL 2敲除菌株。在我们的植物病原系统T。virens是一种比T.深绿色木霉和两种木霉的结果一致地表明，在用sm 2/epl 2敲除菌株处理的植物中，植物保护水平的降低比用sm 1/epl 1敲除菌株处理的更强烈。尽管在真菌生长过程中，角Platanin基因sm 1/epl 1比sm 2/epl 2更丰富地表达，但有趣的是，Sm 2/Epl 2比Sm 1/Epl 1更重要地促进木霉在玉米-C中赋予的植物保护。异株菌致病系统本文的在线版本（doi：10.1186/s12866-014-0333-0）包含补充材料，可供授权用户使用。
The proteins Sm1 and Sm2 from the biocontrol fungus Trichoderma virens belong to the cerato-platanin protein family. Members of this family are small, secreted proteins that are abundantly produced by filamentous fungi with all types of life-styles. Some species of the fungal genus Trichoderma are considered as biocontrol fungi because they are mycoparasites and are also able to directly interact with plants, thereby stimulating plant defense responses. It was previously shown that the cerato-platanin protein Sm1 from T. virens - and to a lesser extent its homologue Epl1 from Trichoderma atroviride - induce plant defense responses. The plant protection potential of other members of the cerato-platanin protein family in Trichoderma, however, has not yet been investigated. In order to analyze the function of the cerato-platanin protein Sm2, sm1 and sm2 knockout strains were generated and characterized. The effect of the lack of Sm1 and Sm2 in T. virens on inducing systemic resistance in maize seedlings, challenged with the plant pathogen Cochliobolus heterostrophus, was tested. These plant experiments were also performed with T. atroviride epl1 and epl2 knockout strains. In our plant-pathogen system T. virens was a more effective plant protectant than T. atroviride and the results with both Trichoderma species showed concordantly that the level of plant protection was more strongly reduced in plants treated with the sm2/epl2 knockout strains than with sm1/epl1 knockout strains. Although the cerato-platanin genes sm1/epl1 are more abundantly expressed than sm2/epl2 during fungal growth, Sm2/Epl2 are, interestingly, more important than Sm1/Epl1 for the promotion of plant protection conferred by Trichoderma in the maize-C. heterostrophus pathosystem. The online version of this article (doi:10.1186/s12866-014-0333-0) contains supplementary material, which is available to authorized users.
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影响因子：
12.3
作者：
Kubicek CP;Herrera-Estrella A;Seidl-Seiboth V;Martinez DA;Druzhinina IS;Thon M;Zeilinger S;Casas-Flores S;Horwitz BA;Mukherjee PK;Mukherjee M;Kredics L;Alcaraz LD;Aerts A;Antal Z;Atanasova L;Cervantes-Badillo MG;Challacombe J;Chertkov O;McCluskey K;Coulpier F;Deshpande N;von Döhren H;Ebbole DJ;Esquivel-Naranjo EU;Fekete E;Flipphi M;Glaser F;Gómez-Rodríguez EY;Gruber S;Han C;Henrissat B;Hermosa R;Hernández-Oñate M;Karaffa L;Kosti I;Le Crom S;Lindquist E;Lucas S;Lübeck M;Lübeck PS;Margeot A;Metz B;Misra M;Nevalainen H;Omann M;Packer N;Perrone G;Uresti-Rivera EE;Salamov A;Schmoll M;Seiboth B;Shapiro H;Sukno S;Tamayo-Ramos JA;Tisch D;Wiest A;Wilkinson HH;Zhang M;Coutinho PM;Kenerley CM;Monte E;Baker SE;Grigoriev IV
通讯作者：
Grigoriev IV
影响因子：
5
作者：
Gaderer, Romana;Bonazza, Klaus;Seidl-Seiboth, Verena
通讯作者：
Seidl-Seiboth, Verena
影响因子：
14.8
作者：
Ram, Arthur F. J.;Klis, Frans M.
通讯作者：
Klis, Frans M.
影响因子：
2.5
作者：
Catalano, Valentina;Vergara, Mariarosaria;Hauzenberger, Jasmin R.;Seiboth, Bernhard;Sarrocco, Sabrina;Vannacci, Giovanni;Kubicek, Christian P.;Seidl-Seiboth, Verena
通讯作者：
Seidl-Seiboth, Verena
影响因子：
2.8
作者：
Vargas, Walter A.;Mukherjee, Prasun K.;Kenerley, Charles M.
通讯作者：
Kenerley, Charles M.