Circ-HIPK3 plays an active role in regulating myoblast differentiation.
Circ-HIPK3 plays an active role in regulating myoblast differentiation.
复制标题
Circ-HIPK3 在调节成肌细胞分化中发挥积极作用。
DOI：
10.1016/j.ijbiomac.2019.11.119
复制
发表时间：
2019-11
影响因子：
8.2
通讯作者：
Hu Shengwei
中科院分区：
文献类型：
--
作者：
Yao Rui;Yao Yang;Li Cunyuan;Li Xiaoyue;Ni Wei;Quan Renzhe;Liu Li;Li Huixiang;Xu Yueren;Zhang Mengdan;Ullah Yaseen;Hu Shengwei
骨骼肌的发育是一个复杂的生物学过程，涉及多种调控途径。深入了解骨骼肌的发育机制对肌肉病变的治疗具有重要意义。近年来的研究表明，环状rna作为一类新的转录后调控因子，在多种生物过程中发挥着重要的调控作用，但其在骨骼肌发育中的功能尚不清楚。我们使用C2C12成肌细胞来研究circ-HIPK3， circ-HIPK3在骨骼肌发育阶段有显著差异，且高表达。我们发现，随着C2C12细胞持续分化，circ-HIPK3的表达水平显著上调（p < 0.05）。结合生物信息学分析和双荧光素酶报告基因实验结果，我们发现circ-HIPK3是miR-124和miR-379调控肌肉分化的海绵。我们的研究表明circ-HIPK3能够促进C2C12成肌细胞的分化，为在circrna水平上进一步研究骨骼肌的发育提供了科学依据。
The development of skeletal muscle is a complex biological process involving a variety of regulatory pathways. A deeper understanding of the developmental mechanisms of skeletal muscle is of great significance for the treatment of muscular lesions. In recent years, studies have shown that circRNAs, as a new class of post-transcriptional regulators, play an important regulatory role in a variety of biological processes, but their function in skeletal muscle development remains unclear. Here, we used C2C12 myoblasts to study circ-HIPK3, which has significant differences in the developmental stages of skeletal muscle and is highly expressed. We found that the expression level of circ-HIPK3 was significantly up-regulated (p < .05) with the persistence of C2C12 cell differentiation. Combining the results of bioinformatics analysis and dual luciferase reporter experiments, we found that circ-HIPK3 is a sponge of miR-124 and miR-379 that regulate muscle differentiation. Our study shows that circ-HIPK3 can promote the differentiation of C2C12 myoblasts, providing a scientific basis for further research on the development of skeletal muscle at the level of circRNAs.
登录
查看更多内容
DOI：
10.1073/pnas.0602831103
发表时间：
2006-06-06
影响因子：
11.1
作者：
Rao, Prakash K.;Kumar, Roshan M.;Lodish, Harvey F.
通讯作者：
Lodish, Harvey F.
影响因子：
--
作者：
Li C;Li X;Yao Y;Ma Q;Ni W;Zhang X;Cao Y;Hazi W;Wang D;Quan R;Hou X;Liu Z;Zhan Q;Liu L;Zhang M;Yu S;Hu S
通讯作者：
Hu S
Sus scrofa 环状 RNA 跨九个器官和三个发育阶段的全基因组分析
DOI：
10.1093/dnares/dsx022
发表时间：
2017-10-01
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Liang G;Yang Y;Niu G;Tang Z;Li K
通讯作者：
Li K
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
影响因子：
2.9
作者：
Bolha L;Ravnik-Glavač M;Glavač D
通讯作者：
Glavač D