Microbial ecology of the murine gut associated with the development of dextran sodium sulfate-induced colitis.
Microbial ecology of the murine gut associated with the development of dextran sodium sulfate-induced colitis.
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DOI：
10.1002/ibd.21462
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发表时间：
2011-04
影响因子：
4.9
通讯作者：
Young, Vincent B.
中科院分区：
文献类型：
--
作者：
Nagalingam, Nabeetha A.;Kao, John Y.;Young, Vincent B.
葡聚糖硫酸钠（DSS）用于诱导小鼠结肠炎。虽然DSS给药引起疾病的确切机制尚不清楚，但有证据表明，常驻细菌在小鼠DSS结肠炎的发展中发挥作用，类似于它们在人类炎症性肠病中的作用。C57 BL/6小鼠接受含5%DSS的饮用水，并在DSS治疗开始后3天和14天实施安乐死。使用非培养物依赖性方法来跟踪DSS处理后肠道微生物群群落结构的变化。疾病的组织学证据和宿主基因表达的变化进行了评估。DSS处理的动物在3天时组织学结肠炎极轻微，但在14天后严重。16 S rRNA编码基因克隆文库的分析表明，DSS处理小鼠盲肠中的微生物群落与对照小鼠中的微生物群落不同。DSS处理的动物盲肠中的微生物群的特征在于微生物丰富度的总体降低、疣微生物门成员的增加和Tenericutes的减少。宿主的炎症反应和微生物群落的变化发生在结肠严重疾病的组织学表现之前，但在盲肠中同时观察到。DSS给药与小鼠肠道微生物多样性的可重复变化相关。微生物和免疫学的变化出现在结肠严重炎症的发展之前。这表明微生物群落的这些变化可能在DSS处理的动物中观察到的异常炎症反应的增强中起作用。
Dextran sodium sulfate (DSS) is used to induce murine colitis. Although the exact mechanism by which DSS administration causes disease is unknown, evidence suggests that the resident bacteria play a role in the development of murine DSS colitis, analogous to their role in human inflammatory bowel diseases. C57BL/6 mice received 5% DSS in the drinking water, and were euthanized three days and 14 days after the initiation of DSS treatment. Culture-independent methods were used to follow changes in the community structure of the gut’s microbiota following DSS treatment. Histologic evidence of disease and changes in host gene expression were assessed. Histologic colitis was minimal in DSS-treated animals at three days, but severe after 14 days. Analysis of 16S rRNA-encoding gene clone libraries demonstrated that the microbial communities in the ceca of DSS-treated mice were distinct from those in control mice. The microbiota in the cecum of DSS-treated animals was characterized by an overall decrease in microbial richness, an increase in members of the phylum Verrucomicrobia, and decrease in Tenericutes. Changes in the host’s inflammatory response and microbial communities occurred before the histologic appearance of severe disease in the colon, but were seen concurrently in the cecum. DSS administration is associated with reproducible changes in the gut microbial diversity of mice. Microbial and immunological changes appeared before the development of severe inflammation in the colon. This indicates that these changes in microbial community may play role in the potentiation of the abnormal inflammatory response seen in DSS-treated animals.
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