Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosome has antineoplastic effects for hepatocellular carcinoma.
Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosome has antineoplastic effects for hepatocellular carcinoma.
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DOI：
10.1371/journal.pone.0183527
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kon M
中科院分区：
文献类型：
--
作者：
Tsuda T;Kaibori M;Hishikawa H;Nakatake R;Okumura T;Ozeki E;Hara I;Morimoto Y;Yoshii K;Kon M
肝癌（HCC）患者的抗癌药物和手术程序已经开发出来，但他们的预后仍然很差。因此，有必要发展新的诊断和治疗策略，以改善其预后。乳糖体是一种核-壳型聚合物胶束，将标记或抗癌药物包封在该胶束中能够实现药物递送。在这项研究中，我们研究了吲哚菁绿色（ICG）负载的乳糖体的近红外荧光（NIF）成像和光动力学治疗（PDT）对肝癌的诊断和治疗效果。用ICG或ICG-乳糖体处理人HCC细胞系HuH-7，然后进行PDT，并测量细胞活力（体外PDT效率）。对于NIF成像，将HuH-7细胞皮下移植到BALB/c裸鼠中，随后静脉内施用ICG或ICG-乳糖体。用PDT处理移植动物，并分析光动力学效应（体内PDT效率）。PDT对ICG-乳糖体处理的HuH-7细胞有毒性作用，但对单独ICG无毒性作用。NIF成像显示，在注射后24小时及其后，ICG-乳糖体处理的动物中肿瘤区域的荧光高于对侧区域的荧光。PDT在ICG-乳糖体处理的移植小鼠中产生立即和持续的光毒性作用。我们的研究结果表明，ICG-乳糖体在异种移植肿瘤中积累，PDT对这些恶性植入物有抑制作用。NIF成像和PDT与ICG-乳糖体可能是有用的诊断和/或治疗肝癌的策略。
Anticancer agents and operating procedures have been developed for hepatocellular carcinoma (HCC) patients, but their prognosis remains poor. It is necessary to develop novel diagnostic and therapeutic strategies for HCC to improve its prognosis. Lactosome is a core-shell-type polymeric micelle, and enclosing labeling or anticancer agents into this micelle enables drug delivery. In this study, we investigated the diagnostic and therapeutic efficacies of indocyanine green (ICG)-loaded lactosome for near-infrared fluorescence (NIF) imaging and photodynamic therapy (PDT) for HCC. The human HCC cell line HuH-7 was treated with ICG or ICG-lactosome, followed by PDT, and the cell viabilities were measured (in vitro PDT efficiency). For NIF imaging, HuH-7 cells were subcutaneously transplanted into BALB/c nude mice, followed by intravenous administration of ICG or ICG-lactosome. The transplanted animals were treated with PDT, and the antineoplastic effects were analyzed (in vivo PDT efficiency). PDT had toxic effects on HuH-7 cells treated with ICG-lactosome, but not ICG alone. NIF imaging revealed that the fluorescence of tumor areas in ICG-lactosome-treated animals was higher than that of contralateral regions at 24 h after injection and thereafter. PDT exerted immediate and continuous phototoxic effects in the transplanted mice treated with ICG-lactosome. Our results demonstrate that ICG-lactosome accumulated in xenograft tumors, and that PDT had antineoplastic effects on these malignant implants. NIF imaging and PDT with ICG-lactosome could be useful diagnostic and/or therapeutic strategies for HCC.
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影响因子：
--
作者：
Funayama, Toru;Sakane, Masataka;Ochiai, Naoyuki
通讯作者：
Ochiai, Naoyuki
DOI：
10.2174/1874120701206010080
发表时间：
2012-01-01
期刊：
The open biomedical engineering journal
影响因子：
--
作者：
Funayama, Toru;Sakane, Masataka;Ochiai, Naoyuki
通讯作者：
Ochiai, Naoyuki
影响因子：
1.6
作者：
Makino, Akira;Yamahara, Ryo;Kimura, Shunsaku
通讯作者：
Kimura, Shunsaku
影响因子：
158.5
作者：
Llovet, Josep M.;Ricci, Sergio;Bruix, Jordi
通讯作者：
Bruix, Jordi
影响因子：
6.2
作者：
Ishizawa, Takeaki;Fukushima, Noriyoshi;Kokudo, Norihiro
通讯作者：
Kokudo, Norihiro