Multiple novel and prevalent astroviruses in pigs.
Multiple novel and prevalent astroviruses in pigs.
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DOI：
10.1016/j.vetmic.2010.11.026
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发表时间：
2011-05-05
影响因子：
3.3
通讯作者：
L'homme Y
中科院分区：
文献类型：
--
作者：
Luo Z;Roi S;Dastor M;Gallice E;Laurin MA;L'homme Y
猪星状病毒多样性和流行病学的知识仍然有限。我们使用了广泛的PCR方法来调查的存在和多样性的星状病毒在不同年龄的健康猪在20个农场和3个屠宰场位于加拿大魁北克省2005年和2007年之间。我们的研究出乎意料地揭示了该省猪的遗传多样性和星状病毒的高流行率。在调查的每个农场和所有年龄组的猪中，从哺乳仔猪到成年猪，都检测到了星状病毒。此外，我们发现近80%的健康育肥猪在屠宰时肠道中含有星状病毒。基于部分聚合酶和完整衣壳序列的系统发育证据表明，猪星状病毒不形成单系群，而是在Mamastrovirus树的不同分支上发现。除了模式种菌株，我们发现高度分歧的菌株，形成两个额外的谱系，其中之福尔斯现有的分类群之外。在大多数健康猪中存在不同的星状病毒可能代表仔猪和可能包括人类在内的其他动物物种的持续感染源。猪星状病毒株似乎不仅与已知的原型人类星状病毒具有遗传学相关性，而且与最近发现的新型人类病毒株也具有遗传学相关性，这表明这些宿主与其他动物物种之间存在多个跨物种传播事件。总的来说，这项研究报告的结果表明，猪在星状病毒科的进化和生态中发挥着积极作用。
Knowledge of porcine astrovirus diversity and epidemiology remains limited. We used a broad range PCR approach to investigate the presence and diversity of astroviruses in healthy pigs of different ages on 20 farms and in 3 slaughterhouses situated in the province of Quebec, Canada between 2005 and 2007. Our study unexpectedly revealed remarkable levels of genetic diversity and high prevalence of astroviruses in pigs of this province. Astroviruses were detected on every farm investigated and in all age groups of pigs, from suckling piglets to adults. In addition, we found that nearly 80% of healthy finisher pigs harbour astroviruses in their intestine at slaughter. Phylogenetic evidence based on partial polymerase and complete capsid sequences, suggests that porcine astroviruses do not form a monophyletic group but are rather found on separate branches across the mamastrovirus tree. In addition to type species strains, we found highly divergent strains that form two additional lineages, one of which falls outside existing taxonomic groups. The presence of diverse astroviruses in a majority of healthy pigs likely represents a continuous source of infection to piglets and possibly to other animal species including humans. Porcine astrovirus strains appeared phylogenetically related not only to prototypical human astroviruses, as was already known, but also to novel human strains recently discovered suggesting multiple cross species transmission events between these hosts and other animal species. Overall, the findings reported in this study suggest an active role of pigs in the evolution and ecology of the Astroviridae.
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影响因子：
2.7
作者：
Akihara, S;Phan, TG;Ushijima, H
通讯作者：
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影响因子：
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BRIDGER, JC;HALL, GA;BROWN, JF
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作者：
Jonassen, CM;Jonassen, TO;Grinde, B
通讯作者：
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