PCB1254 exposure contributes to the abnormalities of optomotor responses and influence of the photoreceptor cell development in zebrafish larvae.
PCB1254 exposure contributes to the abnormalities of optomotor responses and influence of the photoreceptor cell development in zebrafish larvae.
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PCB1254 暴露会导致斑马鱼幼虫视运动反应异常并影响感光细胞发育。
DOI：
10.1016/j.ecoenv.2015.04.026
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
通讯作者）
中科院分区：
文献类型：
--
作者：
Zhang M;Guo XR;Chi X;Tong ML（童梅玲;通讯作者）
多氯联苯(PCbs)是一组剧毒的环境污染物，已被报道会影响儿童的视觉系统发育。然而，潜在的机制尚不清楚。本研究旨在探讨多氯联苯持续暴露对斑马鱼幼体视运动反应(OMR)和视网膜光感受器细胞发育相关基因表达的影响。受精后的斑马鱼胚胎暴露于浓度为0.125、0.25、0.5和1 mg/L的多氯联苯中，直到受精后7天。另设空白对照组和0.01%甲醇对照组。用OMR测试检测视觉行为。用实时定量聚合酶链式反应检测CRX、Rho、SWS1和SWS2的mRNA表达。OMR测试结果表明，当栅格空间频率为0.20Lp/mm、运动速度为25 cm/S时，仔鱼的视觉行为最为敏感，且0.5和1 mg/L PCB1254处理组的正游鱼比例显著低于对照组(P<；0.05)。此外，PCB1254各剂量组的SWS2表达均显著下调(P<0.05)，而PCB1254 0.5和1 mg/100mgPCB1254组的CRX、Rho和SWS1的表达均显著降低(P<；0.05)。这是第一个证明斑马鱼幼体持续暴露于多氯联苯会导致感光细胞发育相关基因表达改变，从而导致OMR行为改变的报告。对这些视觉行为范式的分析可能有助于预测毒物对鱼类视觉功能的不利影响。
Polychlorinated biphenyls (PCBs), a group of highly toxic environmental pollutants, have been report to influence the visual system development in children. However, the underlying mechanism is unclear. The study was aim to investigate the effects of continuous PCBs exposure on optomotor response (OMR) and retinal photoreceptor cell development-related gene expression in zebrafish larvae. The fertilized zebrafish embryos were exposed to PCBs at concentrations of 0.125, 0.25, 0.5, and 1 mg/L until 7 days post-fertilization. Control groups with blank and 0.01% methanol were also prepared. OMR test was used to detect the visual behavior. The mRNA expression of the CRX, RHO, SWS1, and SWS2 was assessed by the Quantitative Real-Time PCR. The OMR test showed that the visual behavior of the larvae was most sensitive when the grating spatial frequency was 0.20 LP/mm and the moving speed was 25 cm/s. Moreover, the proportion of positively swimming fish was significantly reduced in the 0.5 and 1 mg/L PCB1254treatment group (P<0.05) compared with the controls. In addition, the expression of SWS2 was significantly down-regulated in all PCB1254 treatment groups (P<0.05), whereas the decreased expression of the CRX, RHO and SWS1 was found in the 0.5 and 1 mg/L PCB1254 groups (P<0.05). This is the first report to demonstrate that continue exposure of zebrafish larvae to PCBs causes photoreceptor cell development-related gene expression changes that lead to OMR behavioral alterations. Analysis of these visual behavioral paradigms may be useful in predicting the adverse effects of toxicants on visual function in fish.
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影响因子：
4.5
作者：
Tran NM;Zhang A;Zhang X;Huecker JB;Hennig AK;Chen S
通讯作者：
Chen S
影响因子：
1.8
作者：
Abdejalil, J;Hamid, M;Serge, P
通讯作者：
Serge, P
影响因子：
5.1
作者：
Jian Yin;Jian-ming Yang;Feng Zhang;Peng Miao;Yingxin Lin;Mingli Chen
通讯作者：
Jian Yin;Jian-ming Yang;Feng Zhang;Peng Miao;Yingxin Lin;Mingli Chen
DOI：
10.1016/j.scitotenv.2012.07.097
发表时间：
2013-10
期刊：
The Science of the total environment
影响因子：
--
作者：
Tze Wai Wong;A. Wong;E. Anthony S. Nelson;H. Qiu;Susanna Y.K. Ku
通讯作者：
Tze Wai Wong;A. Wong;E. Anthony S. Nelson;H. Qiu;Susanna Y.K. Ku
胚胎暴露于多氯联苯对斑马鱼（Danio rerio）视网膜发育的影响
DOI：
10.1002/jat.1650
发表时间：
2012-03
期刊：
J Appl Toxicol
影响因子：
--
作者：
Qin Hong;*Xia Chia;Chun-Zhao Kou;Mei Guo;Da-ni Qin;Chun-Mei Zhang;Xi-Rong Guo;Mei-Ling Tong;Yan-Ping Wang
通讯作者：
Yan-Ping Wang