An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans.
An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans.
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DOI：
10.3791/52681
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发表时间：
2015-04-09
期刊：
影响因子：
--
通讯作者：
Bettinger JC
中科院分区：
文献类型：
--
作者：
Davies AG;Blackwell GG;Raabe RC;Bettinger JC
酒精使用障碍是一个重大的公共卫生问题，对此几乎没有有效的治疗策略。阻碍更有效治疗方法发展的一个困难是对乙醇对行为影响的分子基础相对缺乏了解。秀丽隐杆线虫（秀丽隐杆线虫）提供了一个有用的模型，用于产生和测试关于乙醇分子效应的假设。在这里，我们描述了一种已经开发并用于检查特定基因和环境因素在乙醇行为反应中的作用的实验，其中运动是行为输出。乙醇剂量依赖性引起在琼脂表面上爬行的急性抑制。效果是动态的；暴露于高浓度的动物表现出最初强烈的爬行抑制，这里称为初始敏感性，然后部分恢复运动速度，尽管药物持续存在。这种乙醇诱导的行为可塑性在这里被称为急性功能耐受性的发展。该分析已被用来证明这两种表型是不同的和遗传上可分离的。这里描述的直接运动试验适用于检查遗传和环境操作对秀丽隐杆线虫对乙醇的这些急性行为反应的影响。
Alcohol use disorders are a significant public health concern, for which there are few effective treatment strategies. One difficulty that has delayed the development of more effective treatments is the relative lack of understanding of the molecular underpinnings of the effects of ethanol on behavior. The nematode, Caenorhabditis elegans (C. elegans), provides a useful model in which to generate and test hypotheses about the molecular effects of ethanol. Here, we describe an assay that has been developed and used to examine the roles of particular genes and environmental factors in behavioral responses to ethanol, in which locomotion is the behavioral output. Ethanol dose-dependently causes an acute depression of crawling on an agar surface. The effects are dynamic; animals exposed to a high concentration demonstrate an initial strong depression of crawling, referred to here as initial sensitivity, and then partially recover locomotion speed despite the continued presence of the drug. This ethanol-induced behavioral plasticity is referred to here as the development of acute functional tolerance. This assay has been used to demonstrate that these two phenotypes are distinct and genetically separable. The straightforward locomotion assay described here is suitable for examining the effects of both genetic and environmental manipulations on these acute behavioral responses to ethanol in C. elegans.
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影响因子：
3.7
作者：
Topper SM;Aguilar SC;Topper VY;Elbel E;Pierce-Shimomura JT
通讯作者：
Pierce-Shimomura JT
影响因子：
3.7
作者：
Schuckit, MA
通讯作者：
Schuckit, MA
影响因子：
2.8
作者：
Mitchell, P. H.;Bull, K.;O'Connor, V.
通讯作者：
O'Connor, V.
影响因子：
4.2
作者：
Davies, Andrew G.;Friedberg, Ryan I.;Bettinger, Jill C.
通讯作者：
Bettinger, Jill C.
影响因子：
5.3
作者：
Davis, Scott J.;Scott, Luisa L.;Pierce-Shimomura, Jonathan T.
通讯作者：
Pierce-Shimomura, Jonathan T.