Diverse GABAergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleus.
Diverse GABAergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleus.
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DOI：
10.1111/jnc.15101
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发表时间：
2021-11
影响因子：
4.7
通讯作者：
Fox MA
中科院分区：
文献类型：
--
作者：
Sabbagh U;Govindaiah G;Somaiya RD;Ha RV;Wei JC;Guido W;Fox MA
在视觉系统中，视网膜轴突将来自外部世界的视觉信息传递到数十个不同的视网膜受体脑区域，并在多个水平上组织该信息，包括视网膜传入水平，内在视网膜受体神经元的细胞结构，或两者的组合。由视网膜轴突密集支配的两个主要的视网膜受体核是背外侧膝状体核，其对于经典的图像形成视觉是重要的，以及腹侧LGN（vLGN），其与非图像形成视觉相关。vLGN中的神经化学、细胞结构和视网膜丘脑连接性仍未得到解决，提出了它如何接收和处理视觉信息的基本问题。为了阐明这些重要的问题，使用原位杂交，免疫组织化学和遗传报告细胞系来识别和表征小鼠vLGN中的新型神经元细胞类型。不仅这些细胞的高百分比是GABA能的，我们还发现转录组学上不同的GABA能细胞类型存在于vLGN的两个主要层中，即视网膜受体、外部vLGN（vLGNe）和非视网膜受体、内部vLGN（vLGNi）。此外，在vLGNe中，我们确定了分布在四个相邻亚板层中的GABA能细胞的转录不同亚型。使用跨突触病毒追踪和体外电生理学，我们发现每个这些vLGNe亚板层中的细胞接收来自视网膜的单突触输入。这些结果不仅确定了vLGN中GABA能细胞的新亚型，而且表明vLGNe中视网膜受体细胞的亚型特异性层状分布可能对皮层下视觉系统平行通道中接收、处理和传输光源信号很重要。腹外侧膝状体核（vLGN）是视丘脑的一部分。它可以广泛地分为两个结构域或层，外部vLGNe（其接收视网膜输入）和内部vLGNi（不接收视网膜输入）。在这项研究中，我们描述了转录组不同的GABA能神经元，填充vLGN和组织成离散的，相邻的亚板层中的vLGNe亚型。总之，我们的结果显示vLGNe中视网膜受体神经元的四个亚型特异性亚层。
In the visual system, retinal axons convey visual information from the outside world to dozens of distinct retinorecipient brain regions and organize that information at several levels, including either at the level of retinal afferents, cytoarchitecture of intrinsic retinorecipient neurons, or a combination of the two. Two major retinorecipient nuclei which are densely innervated by retinal axons are the dorsal lateral geniculate nucleus, which is important for classical image‐forming vision, and ventral LGN (vLGN), which is associated with non‐image‐forming vision. The neurochemistry, cytoarchitecture, and retinothalamic connectivity in vLGN remain unresolved, raising fundamental questions of how it receives and processes visual information. To shed light on these important questions, used in situ hybridization, immunohistochemistry, and genetic reporter lines to identify and characterize novel neuronal cell types in mouse vLGN. Not only were a high percentage of these cells GABAergic, we discovered transcriptomically distinct GABAergic cell types reside in the two major laminae of vLGN, the retinorecipient, external vLGN (vLGNe) and the non‐retinorecipient, internal vLGN (vLGNi). Furthermore, within vLGNe, we identified transcriptionally distinct subtypes of GABAergic cells that are distributed into four adjacent sublaminae. Using trans‐synaptic viral tracing and in vitro electrophysiology, we found cells in each these vLGNe sublaminae receive monosynaptic inputs from retina. These results not only identify novel subtypes of GABAergic cells in vLGN, they suggest the subtype‐specific laminar distribution of retinorecipient cells in vLGNe may be important for receiving, processing, and transmitting light‐derived signals in parallel channels of the subcortical visual system. The ventral lateral geniculate nucleus (vLGN) is part of the visual thalamus. It can broadly be separated into two structural domains or laminae, the external vLGNe (which receives retinal input) and the internal vLGNi (receives no retinal input). In this study, we describe subtypes of transcriptomically distinct GABAergic neurons that populate the vLGN and organize into discrete, adjacent sublaminae in the vLGNe. Taken together, our results show four subtype‐specific sublaminae of retinorecipient neurons in vLGNe.
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影响因子：
2.9
作者：
Evangelio M;García-Amado M;Clascá F
通讯作者：
Clascá F
影响因子：
25
作者：
Gouwens NW;Sorensen SA;Berg J;Lee C;Jarsky T;Ting J;Sunkin SM;Feng D;Anastassiou CA;Barkan E;Bickley K;Blesie N;Braun T;Brouner K;Budzillo A;Caldejon S;Casper T;Castelli D;Chong P;Crichton K;Cuhaciyan C;Daigle TL;Dalley R;Dee N;Desta T;Ding SL;Dingman S;Doperalski A;Dotson N;Egdorf T;Fisher M;de Frates RA;Garren E;Garwood M;Gary A;Gaudreault N;Godfrey K;Gorham M;Gu H;Habel C;Hadley K;Harrington J;Harris JA;Henry A;Hill D;Josephsen S;Kebede S;Kim L;Kroll M;Lee B;Lemon T;Link KE;Liu X;Long B;Mann R;McGraw M;Mihalas S;Mukora A;Murphy GJ;Ng L;Ngo K;Nguyen TN;Nicovich PR;Oldre A;Park D;Parry S;Perkins J;Potekhina L;Reid D;Robertson M;Sandman D;Schroedter M;Slaughterbeck C;Soler-Llavina G;Sulc J;Szafer A;Tasic B;Taskin N;Teeter C;Thatra N;Tung H;Wakeman W;Williams G;Young R;Zhou Z;Farrell C;Peng H;Hawrylycz MJ;Lein E;Ng L;Arkhipov A;Bernard A;Phillips JW;Zeng H;Koch C
通讯作者：
Koch C
影响因子：
2.5
作者：
GODEMENT, P;SALAUN, J;IMBERT, M
通讯作者：
IMBERT, M
影响因子：
3.6
作者：
Hammer, Sarah;Carrillo, Gabriela L.;Fox, Michael A.
通讯作者：
Fox, Michael A.
影响因子：
5.7
作者：
Dhande OS;Huberman AD
通讯作者：
Huberman AD