Improved method of ink-gelatin perfusion for visualising rat retinal microvessels.
Improved method of ink-gelatin perfusion for visualising rat retinal microvessels.
复制标题
改进的墨水明胶灌注方法用于可视化大鼠视网膜微血管。
DOI：
10.1267/ahc.08015
复制
发表时间：
2008-10-29
影响因子：
2.4
通讯作者：
Xuegang L
中科院分区：
文献类型：
--
作者：
Jianbin T;Liang H;Jufang H;Hui W;Dan C;Leping Z;Jin Z;Xuegang L
为了完全可视化大鼠视网膜微血管，使用血管性血友病因子（vWf）免疫染色作为对照，系统优化了明胶墨水灌注条件。同时，对新的灌注条件下显示的血管是否可以与同一视网膜内的神经元或胶质细胞进行双标记进行了测试。我们的结果表明，首先在140%大鼠平均动脉压（MAP）下用20 ml 37°C墨水加3%明胶输注大鼠，随后在180%大鼠MAP下用20 ml 37°C墨水加5%明胶输注大鼠，允许墨水完全填充大鼠视网膜微血管。灌注法标记的大鼠视网膜微血管数量多于vWf免疫组化法。此外，我们的数据首次显示，改进的明胶墨水灌注对同一组织中视网膜神经元或胶质细胞的荧光金标记或免疫染色没有影响，也没有造成污染。这些数据表明，改进的明胶墨水灌注技术是一种上级的大鼠视网膜微血管的形态学表征的方法，兼容的胶质细胞和神经元的双重标记，它扩展了经典方法的实用规模。
To visualize completely rat retinal microvessels, the gelatin-ink perfusion condition was systematically optimized using von Willebrand factor (vWf) immunostaining as control. Whether the vessel showed by the new perfusion condition can be used for double label with neurons or glial cells in the same retina was also tested. Our results showed that infusing rats first with 20 ml of 37°C ink plus 3% gelatin at 140% rat mean arterial pressure (MAP), and subsequently with 20 ml of 37°C ink plus 5% gelatin at 180% rat MAP allowed the ink to completely fill the rat retinal microvessels. Rat retinal microvessels labeled by the perfusion method were more in number than that by vWf immunostaining. Moreover, our data, for the first time, displayed that the improved gelatin-ink perfusion had no effect on and caused no contamination to the following fluorogold labeling or immunostaining of retinal neurons or glial cells in the same tissue. These data suggest that the improved gelatin-ink perfusion technique is a superior method for morphological characterization of rat retinal microvessels, compatible to the double labeling of glial cells and neurons, and it extends the practical scale of the classic method.
登录
查看更多内容
影响因子：
4.2
作者：
Kalinin, Sergey;Gavrilyuk, Vitaliy;Feinstein, Douglas L.
通讯作者：
Feinstein, Douglas L.
影响因子：
3.1
作者：
BELL, MA;SCARROW, WG
通讯作者：
SCARROW, WG
影响因子：
3
作者：
Tata, DA;Anderson, BJ
通讯作者：
Anderson, BJ
影响因子：
4.1
作者：
Barou, O;Mekraldi, S;Lafage-Proust, MH
通讯作者：
Lafage-Proust, MH
利钠肽定位于大鼠视网膜无长突细胞。
影响因子：
2.5
作者：
Jin, Ying;Zhong, Yong-Mei;Yang, Xiong-Li
通讯作者：
Yang, Xiong-Li