Molecular characterization of the small nonstructural proteins of parvovirus Aleutian mink disease virus (AMDV) during infection.
Molecular characterization of the small nonstructural proteins of parvovirus Aleutian mink disease virus (AMDV) during infection.
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DOI：
10.1016/j.virol.2014.01.005
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发表时间：
2014-03
期刊：
影响因子：
3.7
通讯作者：
Qiu, Jianming
中科院分区：
文献类型：
--
作者：
Huang, Qinfeng;Luo, Yong;Cheng, Fang;Best, Sonja M.;Bloom, Marshall E.;Qiu, Jianming
阿留申水貂病病毒（AMDV）是细小病毒科Amdovirus属唯一的成员。在AMDV感染过程中，一个前体mRNA通过选择性剪接和选择性聚腺苷酸化产生六种病毒转录物。除了大的非结构蛋白NS1外，两种小的非结构蛋白NS2和NS3也被推测编码（Qiu J, et al: Journal of Virology, 80:654 - 62,2006）。然而，这两种蛋白在病毒感染过程中尚未得到实验证明，对其功能也一无所知。本研究研究了AMDV的非结构蛋白表达谱，首次证实了感染过程中NS2和NS3的表达，并确定了它们在细胞内的定位。更重要的是，我们提供的证据表明，NS2和NS3都是AMDV复制所必需的。
Aleutian mink disease virus (AMDV) is the only member in genus Amdovirus of the family Parvoviridae. During AMDV infection, six species of viral transcripts are generated from one precursor mRNA through alternative splicing and alternative polyadenylation. In addition to the large non-structural protein NS1, two small non-structural proteins, NS2 and NS3, are putatively encoded (Qiu J, et al: Journal of Virology, 80:654–62, 2006). However, these two proteins have not been experimentally demonstrated during virus infection, and nothing is known about their function. Here, we studied the nonstructural protein expression profile of AMDV, and for the first time, confirmed expression of NS2 and NS3 during infection, and identified their intracellular localization. More importantly, we provided evidence that both NS2 and NS3 are necessary for AMDV replication.
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