Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
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DOI：
10.1016/j.chom.2017.12.018
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发表时间：
2018-02-14
影响因子：
30.3
通讯作者：
Dong M
中科院分区：
文献类型：
--
作者：
Zhang S;Lebreton F;Mansfield MJ;Miyashita SI;Zhang J;Schwartzman JA;Tao L;Masuyer G;Martínez-Carranza M;Stenmark P;Gilmore MS;Doxey AC;Dong M
肉毒神经毒素（Botanicneurotoxins，BoNTs）是由梭菌属（Clostridium）产生的一类具有生物恐怖作用的细菌毒素。在这里，我们报告说，屎肠球菌菌株从奶牛粪便中分离携带BoNT样毒素，指定BoNT/En。它切割VAMP 2和SNAP-25，这两种蛋白质介导神经元中突触囊泡胞吐作用，切割位点与这两种蛋白质上已知的BoNT切割位点不同。比较基因组分析表明，E.携带BoNT/En的屎肠菌菌株是大肠杆菌型的，BoNT/En基因位于206-kb pueruginosa接合质粒上的典型BoNT基因簇内。虽然BoNT/En靶向的宿主物种仍有待确定，但这些发现确立了BoNT的扩展成员，并证明了E.屎肠球菌，一种在人类和动物中普遍存在的寄生生物，是医院获得性多药耐药（MDR）感染的主要原因，水平获得并可能传播独特的BoNT基因簇。肉毒杆菌神经毒素（BoNTs）是由梭菌属中的多种细菌产生的强效毒素。Zhang等报道了屎肠球菌的一种肠球菌菌株携带编码BoNT样毒素基因的接合质粒。因此，一种寄生生物可以获得并可能传播BoNT基因。
Botulinum neurotoxins (BoNTs), produced by various Clostridium strains, are a family of potent bacterial toxins and potential bioterrorism agents. Here we report that an Enterococcus faecium strain isolated from cow feces carries a BoNT-like toxin, designated BoNT/En. It cleaves both VAMP2 and SNAP-25, proteins that mediate synaptic vesicle exocytosis in neurons, at sites distinct from known BoNT cleavage sites on these two proteins. Comparative genomic analysis determines that the E. faecium strain carrying BoNT/En is a commensal-type, and the BoNT/En gene is located within a typical BoNT gene cluster on a 206-kb putatively conjugative plasmid. Although the host species targeted by BoNT/En remains to be determined, these findings establish an extended member of BoNTs and demonstrate the capability of E. faecium, a commensal organism ubiquitous in humans and animals and a leading cause of hospital-acquired multidrug resistant (MDR) infections, to horizontally acquire, and possibly disseminate, a unique BoNT gene cluster. Botulinum neurotoxins (BoNTs) are potent toxins produced by diverse bacteria in the Clostridium genus. Zhang et al. report that a commensal strain of Enterococcus faecium carries a conjugative plasmid encoding a BoNT-like toxin gene. Thus, a commensal organism can acquire and possibly disseminate BoNT genes.
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影响因子：
64.5
作者：
Lebreton F;Manson AL;Saavedra JT;Straub TJ;Earl AM;Gilmore MS
通讯作者：
Gilmore MS
影响因子：
7.4
作者：
Kalb, Suzanne R.;Baudys, Jakub;Barr, John R.
通讯作者：
Barr, John R.
影响因子：
6.4
作者：
Montecucco C;Rasotto MB
通讯作者：
Rasotto MB
影响因子：
6.4
作者：
Maslanka, Susan E.;Luquez, Carolina;Johnson, Eric A.
通讯作者：
Johnson, Eric A.
DOI：
10.1126/science.1214270
发表时间：
2012-02-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gu S;Rumpel S;Zhou J;Strotmeier J;Bigalke H;Perry K;Shoemaker CB;Rummel A;Jin R
通讯作者：
Jin R