In planta function of compatible solute transporters of the AtProT family.
In planta function of compatible solute transporters of the AtProT family.
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DOI：
10.1093/jxb/erq320
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发表时间：
2011-01
影响因子：
6.9
通讯作者：
Rentsch D
中科院分区：
文献类型：
--
作者：
Lehmann S;Gumy C;Blatter E;Boeffel S;Fricke W;Rentsch D
拟南芥（Arabidopsis thaliana）的三种脯氨酸转运蛋白（AtProTs）在异种体系中表达时可转运相容的溶质脯氨酸和甘氨酸甜菜碱以及胁迫诱导的化合物γ-氨基丁酸。本研究的目的是显示这三种AtProTs在植物中的转运和生理相关性。利用单、双、三重敲除突变体和AtProT过表达系，分析了脯氨酸含量、脯氨酸生长、放射性标记甜菜碱的运输、AtProT基因的表达和脯氨酸代谢酶的表达。研究表明，AtProT2可以促进植物对L-和d -脯氨酸以及[14C]甘氨酸甜菜碱的吸收，这表明它在向根部输入相容溶质方面发挥了作用。L-和d -脯氨酸的毒性浓度导致atprot过表达植物的生长严重迟缓，表明需要精确调节脯氨酸的摄取和/或分布。此外，有证据表明，AtProT基因在脯氨酸含量升高的组织中高度表达，即花粉和叶表皮。
The three proline transporters of Arabidopsis thaliana (AtProTs) transport the compatible solutes proline and glycine betaine and the stress-induced compound γ-aminobutyric acid when expressed in heterologous systems. The aim of the present study was to show transport and physiological relevance of these three AtProTs in planta. Using single, double, and triple knockout mutants and AtProT-overexpressing lines, proline content, growth on proline, transport of radiolabelled betaine, and expression of AtProT genes and enzymes of proline metabolism were analysed. AtProT2 was shown to facilitate uptake of L- and D-proline as well as [14C]glycine betaine in planta, indicating a role in the import of compatible solutes into the root. Toxic concentrations of L- and D-proline resulted in a drastic growth retardation of AtProT-overexpressing plants, demonstrating the need for a precise regulation of proline uptake and/or distribution. Furthermore evidence is provided that AtProT genes are highly expressed in tissues with elevated proline content—that is, pollen and leaf epidermis.
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