A PPR Protein ACM1 Is Involved in Chloroplast Gene Expression and Early Plastid Development in Arabidopsis.
A PPR Protein ACM1 Is Involved in Chloroplast Gene Expression and Early Plastid Development in Arabidopsis.
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DOI：
10.3390/ijms22052512
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发表时间：
2021-03-03
影响因子：
5.6
通讯作者：
Xiao J
中科院分区：
文献类型：
--
作者：
Wang X;An Y;Li Y;Xiao J
叶绿体的正常发育离不开细胞核和叶绿体基因组之间各种蛋白质和信号联系的协调作用。关于这些进程的许多问题仍然没有答案。在这里，我们报告了一个新的P型五肽重复（PPR）因子，命名为白化病子叶突变体1（ACM 1），这是由一个核基因编码，并参与叶绿体发育。敲除ACM 1基因的转基因植物显示出白化病子叶，但正常的真叶，而在幼苗中敲除ACM 1基因是致命的。荧光蛋白分析表明，ACM 1是专门定位在叶绿体。PEP依赖的质体转录水平和剪接效率的几个第二组内含子在子叶中的RNAi线受到严重影响。此外，变性凝胶电泳和蛋白质印迹实验表明，叶绿体核糖体的积累可能受到破坏。总之，我们的研究结果表明，ACM 1是不可缺少的早期叶绿体发育在拟南芥子叶。
Chloroplasts cannot develop normally without the coordinated action of various proteins and signaling connections between the nucleus and the chloroplast genome. Many questions regarding these processes remain unanswered. Here, we report a novel P-type pentatricopeptide repeat (PPR) factor, named Albino Cotyledon Mutant1 (ACM1), which is encoded by a nuclear gene and involved in chloroplast development. Knock-down of ACM1 transgenic plants displayed albino cotyledons but normal true leaves, while knock-out of the ACM1 gene in seedlings was lethal. Fluorescent protein analysis showed that ACM1 was specifically localized within chloroplasts. PEP-dependent plastid transcript levels and splicing efficiency of several group II introns were seriously affected in cotyledons in the RNAi line. Furthermore, denaturing gel electrophoresis and Western blot experiments showed that the accumulation of chloroplast ribosomes was probably damaged. Collectively, our results indicate ACM1 is indispensable in early chloroplast development in Arabidopsis cotyledons.
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