Simple observation of Streptococcus mutans biofilm by scanning electron microscopy using ionic liquids.
Simple observation of Streptococcus mutans biofilm by scanning electron microscopy using ionic liquids.
复制标题
DOI：
10.1186/s13568-015-0097-4
复制
发表时间：
2015-12
期刊：
影响因子：
3.7
通讯作者：
Hayashi M
中科院分区：
文献类型：
--
作者：
Asahi Y;Miura J;Tsuda T;Kuwabata S;Tsunashima K;Noiri Y;Sakata T;Ebisu S;Hayashi M
扫描电子显微镜（SEM）由于其高分辨率和放大倍数，已成功地用于生物膜成像。然而，传统的SEM需要在观察之前对生物样品进行脱水和金属涂覆，并且由于生物膜主要由水组成，样品脱水可能会影响生物膜结构。当涂覆有离子液体（其是一种在室温下以液态存在的盐）时，用于SEM观察的生物样品不需要脱水或金属涂覆，因为离子液体在真空条件下不蒸发并且是导电的。本研究探讨了离子液体的能力，使变形链球菌生物膜的扫描电镜观察相比，传统的涂层方法。使用两种亲水性和两种疏水性离子液体，它们都是电子导体。与常规方法制备的样品相比，离子液体处理的样品不表现出纤维状细胞外基质结构和生物膜表面上的开裂。亲水性离子液体比疏水性离子液体能更清晰地显示生物膜结构。这项研究发现，离子液体是有用的，允许通过SEM观察生物膜，而无需通过脱水和金属涂层制备。本文的在线版本（doi：10.1186/s13568-015-0097-4）包含补充材料，可供授权用户使用。
Scanning electron microscopy (SEM) has been successfully used to image biofilms because of its high resolution and magnification. However, conventional SEM requires dehydration and metal coating of biological samples before observation, and because biofilms consist mainly of water, sample dehydration may influence the biofilm structure. When coated with an ionic liquid, which is a kind of salt that exists in the liquid state at room temperature, biological samples for SEM observation do not require dehydration or metal coating because ionic liquids do not evaporate under vacuum conditions and are electrically conductive. This study investigates the ability of ionic liquids to allow SEM observation of Streptococcus mutans biofilms compared with conventional coating methods. Two hydrophilic and two hydrophobic ionic liquids, all of which are electronic conductors, are used. Compared with samples prepared by the conventional method, the ionic-liquid-treated samples do not exhibit a fibrous extracellular matrix structure and cracking on the biofilm surface. The hydrophilic ionic liquids give clearer images of the biofilm structure than those of the hydrophobic ionic liquids. This study finds that ionic liquids are useful for allowing the observation of biofilms by SEM without preparation by dehydration and metal coating. The online version of this article (doi:10.1186/s13568-015-0097-4) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.6
作者：
Boles, BR;Thoendel, M;Singh, PK
通讯作者：
Singh, PK
影响因子：
9.9
作者：
Brown, Paul;Butts, Craig P.;Khan, Asad
通讯作者：
Khan, Asad
影响因子：
2.5
作者：
Ishigaki, Yasuhito;Nakamura, Yuka;Kuwabata, Susumu
通讯作者：
Kuwabata, Susumu
影响因子：
4.3
作者：
Noiri, Y;Okami, Y;Ebisu, S
通讯作者：
Ebisu, S
影响因子：
--
作者：
Alhede, Morten;Qvortrup, Klaus;Bjarnsholt, Thomas
通讯作者：
Bjarnsholt, Thomas