A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells.
A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells.
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DOI：
10.1016/j.dental.2012.08.002
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发表时间：
2013-01
期刊：
影响因子：
5
通讯作者：
Noer, Jacques E.
中科院分区：
文献类型：
--
作者：
Cavalcanti, Bruno N.;Zeitlin, Benjamin D.;Noer, Jacques E.
干细胞再生牙髓的临床翻译需要进一步开发合适的可注射支架。Peramatrix™是一种在正常生理条件下瞬间聚合的确定的、自组装的多肽水凝胶。在这里，我们评估了Puramatrix™与牙髓干细胞生长和分化的相容性。DPSC细胞在0.05%~0.25%的™中培养。用WST-1比色法测定细胞存活率。用共聚焦显微镜观察细胞的三维形态。此外，我们使用了含有PuramatrixDPSC的人牙齿切片模型来验证™向成牙本质细胞样细胞的分化，这是通过检测DSPP和DMP-1的表达来衡量的。在™中培养至少3周后，DPSC存活并增殖。共聚焦显微镜观察发现，接种在™中的细胞具有健康细胞的形态特征，部分细胞出现胞质伸长。值得注意的是，在含有Puramatrix™的牙齿切片中，21天后，DPSC细胞表达DMP-1和DSPP，这是可能的成牙本质细胞分化的标志。总而言之，这些数据表明，自组装的多肽水凝胶可能是干细胞再生牙本质的有用的可注射支架。
The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. DPSC cells were grown in 0.05 to 0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3-D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics.
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