Contrasting genes conferring short- and long-term biofilm adaptation in Listeria.
Contrasting genes conferring short- and long-term biofilm adaptation in Listeria.
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DOI：
10.1099/mgen.0.001114
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发表时间：
2023-10
影响因子：
3.9
通讯作者：
Sheppard SK
中科院分区：
文献类型：
--
作者：
Monteith W;Pascoe B;Mourkas E;Clark J;Hakim M;Hitchings MD;McCarthy N;Yahara K;Asakura H;Sheppard SK
单核细胞增生李斯特菌 是一种机会性食源性细菌，能够感染人类，导致高住院率和死亡率。自然人群的基因型和表型是可变的，一些谱系负责大多数人类感染。的成功 L.单核 与它在食物和环境中的生存能力有关。生物膜是使这些细菌持续存在并感染人类的重要特征，因此了解生物膜形成的遗传基础是了解传播的关键。我们试图研究生物膜形成能力的 L.单核 通过使用全基因组关联研究（GWAS）确定自然种群中生物膜形成的遗传变异。然后使用RNA测序（RNA-seq）研究生物膜形成期间特定菌株的基因表达的变化。通过GWAS在273个基因中鉴定出与生物膜形成增强相关的遗传变异，并通过RNA-seq在220个基因中鉴定出差异表达。统计分析表明，任何一种类型的实验标记的重叠基因的数量比随机抽样的预期要少。这一新发现与进化情景一致，即先驱基因的基因表达变异驱动快速适应，随后核心基因组内的核苷酸变化驱动较慢的适应。
Listeria monocytogenes is an opportunistic food-borne bacterium that is capable of infecting humans with high rates of hospitalization and mortality. Natural populations are genotypically and phenotypically variable, with some lineages being responsible for most human infections. The success of L. monocytogenes is linked to its capacity to persist on food and in the environment. Biofilms are an important feature that allow these bacteria to persist and infect humans, so understanding the genetic basis of biofilm formation is key to understanding transmission. We sought to investigate the biofilm-forming ability of L. monocytogenes by identifying genetic variation that underlies biofilm formation in natural populations using genome-wide association studies (GWAS). Changes in gene expression of specific strains during biofilm formation were then investigated using RNA sequencing (RNA-seq). Genetic variation associated with enhanced biofilm formation was identified in 273 genes by GWAS and differential expression in 220 genes by RNA-seq. Statistical analyses show that the number of overlapping genes flagged by either type of experiment is less than expected by random sampling. This novel finding is consistent with an evolutionary scenario where rapid adaptation is driven by variation in gene expression of pioneer genes, and this is followed by slower adaptation driven by nucleotide changes within the core genome.
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影响因子：
3.9
作者：
Aidley J;Wanford JJ;Green LR;Sheppard SK;Bayliss CD
通讯作者：
Bayliss CD
与生物膜形成负相关的嗜水气单胞菌寡肽酶基因 pepF 的鉴定和表征
影响因子：
5.2
作者：
Du H;Pang M;Dong Y;Wu Y;Wang N;Liu J;Awan F;Lu C;Liu Y
通讯作者：
Liu Y
影响因子：
4.5
作者：
Colagiorgi A;Di Ciccio P;Zanardi E;Ghidini S;Ianieri A
通讯作者：
Ianieri A
影响因子：
6.4
作者：
Brockman KL;Azzari PN;Branstool MT;Atack JM;Schulz BL;Jen FE;Jennings MP;Bakaletz LO
通讯作者：
Bakaletz LO
影响因子：
3.3
作者：
Bucher, Tabitha;Oppenheimer-Shaanan, Yaara;Kolodkin-Gal, Ilana
通讯作者：
Kolodkin-Gal, Ilana