The in vivo reutilization of lymphocytic and sarcoma DNA by cells growing in the peritoneal cavity.
The in vivo reutilization of lymphocytic and sarcoma DNA by cells growing in the peritoneal cavity.
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在腹膜腔中生长的细胞对淋巴细胞和肉瘤DNA的体内重新化。
DOI：
10.1083/jcb.13.2.205
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发表时间：
1962-05
影响因子：
7.8
通讯作者：
RIEKE, WO
中科院分区：
文献类型：
--
作者：
RIEKE, WO
将腹水肿瘤肉瘤I移植到1至24小时前用氚化胸苷标记的同源和同源小鼠中。放射自显影制剂显示标记的宿主淋巴细胞与移植的肿瘤混合，随后肉瘤中出现核放射性。在先前标记的小鼠中皮下或微孔扩散室中培养的肉瘤细胞没有表现出明显的放射性。将经过清洗的 H3-胸苷标记的淋巴细胞移植到非放射性、携带肉瘤的小鼠中，随后肉瘤中逐渐出现核放射性。肉瘤中的标记被脱氧核糖核酸酶完全去除，但放射自显影前的核糖核酸酶处理却没有去除。在正常或荷瘤小鼠腹腔内注射纯化的、H3-胸苷标记的肉瘤或淋巴DNA，随后肉瘤或正常腹膜单核细胞中出现放射性。结论是DNA及其代谢物可能在体内发生再利用，并讨论了可以检测到再利用的条件。
An ascites tumor, Sarcoma I, was transplanted to isologous and homologous mice which had been labeled with tritiated thymidine from 1 to 24 hours previously. Radioautographic preparations revealed labeled host lymphocytes emerging to mingle with the transplanted tumor and the subsequent appearance of nuclear radioactivity in the sarcoma. Sarcoma cells cultured subcutaneously or in Millipore diffusion chambers in previously labeled mice did not demonstrate significant radioactivity. Transplantation of washed, H3-thymidine-labeled lymphocytes to non-radioactive, sarcoma-bearing mice was followed by the gradual appearance of nuclear radioactivity in the sarcoma. The label in the sarcoma was entirely removed by deoxyribonuclease but not by ribonuclease treatment prior to radioautography. Intraperitoneal injections of purified, H3-thymidine-labeled sarcoma or lymphoid DNA in normal or tumor-bearing mice were followed by radioactivity appearing in sarcoma or normal peritoneal mononuclear cells. It was concluded that reutilization of DNA and its metabolites may occur in vivo, and the conditions under which reutilization may be detected are discussed.
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影响因子：
--
作者：
MESSIER, B;LEBLOND, CP
通讯作者：
LEBLOND, CP
DOI：
10.1083/jcb.9.1.81
发表时间：
1961-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
BORENFREUND, E;BENDICH, A
通讯作者：
BENDICH, A
DOI：
10.1111/j.1749-6632.1959.tb36943.x
发表时间：
1959-01-01
期刊：
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES
影响因子：
--
作者：
CRONKITE, EP;FLIEDNER, TM;QUASTLER, H
通讯作者：
QUASTLER, H
DOI：
10.1016/0006-291x(60)90206-0
发表时间：
1960-01-01
影响因子：
3.1
作者：
GARTLER, SM
通讯作者：
GARTLER, SM
影响因子：
3.7
作者：
HILL, M;DRASIL, V
通讯作者：
DRASIL, V