Shen-Ling-Bai-Zhu-San (SL) and SL Derived-Polysaccharide (PL) Ameliorate the Severity of Diarrhea-Induced by High Lactose via Modification of Colonic Fermentation.
Shen-Ling-Bai-Zhu-San (SL) and SL Derived-Polysaccharide (PL) Ameliorate the Severity of Diarrhea-Induced by High Lactose via Modification of Colonic Fermentation.
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DOI：
10.3389/fphar.2022.883355
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发表时间：
2022
影响因子：
5.6
通讯作者：
Tang, Xudong
中科院分区：
文献类型：
--
作者：
Xue, Hong;Ma, Jinxin;Wang, Yitian;Lu, Mengxiong;Wang, Fengyun;Tang, Xudong
关键词：
在我们前期的研究中，我们证明了经典的中草药神灵白术散(SL)可以缓解乳糖所致的腹泻。然而，关于SL作用的机制或SL衍生的多糖(PL)的疗效，人们知之甚少。在本研究中，我们利用16S rRNA分析方法研究了SL和PL对高乳糖饮食大鼠肠道和粘膜微生物区系失调的改善作用。测定盲肠组织中乳糖、乳酸及盲肠和门静脉组织中短链脂肪酸(SCFAs)的含量，同时测定离子转运体的表达。我们的数据表明，SL、PL和盲肠微生物区系移植(CMT)显著减少了粪便水分含量和水分摄入量。腔内微生物区系中阿克曼属、双歧杆菌和布鲁氏菌的数量显著增加，乳杆菌、志贺氏菌和杜伯氏菌的丰度较低，而粘膜微生物区系中双歧杆菌、阿克曼属、Albaculum、Bilophila和Coribacteriaceae_UCG-002的丰度显著增加，肠球菌、螺杆菌、杜伯氏菌和柯林斯氏菌的丰度较低。此外，处理促进了乳糖发酵和单链脂肪酸的产生，这可能与调节管腔微生物群落有关。与模型组比较，治疗组Na/H交换器3/Na/H交换器3(pNHE3/NHE3)的磷酸化比率降低，单羧酸钠1(SMCT1)的表达增加，这可能与黏膜微生物群落的变化有关。此外，这些治疗可能会恢复受影响的肠道微生物区系的代谢途径。这些结果为SL的作用机制和开发以PL为基础的治疗乳糖性腹泻药物提供了重要依据。
In our previous study, we demonstrated that Shen-ling-bai-zhu-san (SL), a classical Chinese herbal formula, could alleviate lactose-induced diarrhea. However, little is known about the mechanism underlying SL action or the efficacy of the polysaccharide (PL) derived from SL. In this study, we investigated the effect of SL and PL on improving the dysregulated luminal and mucosal microbiota in rats with high lactose diet using 16S rRNA analysis. The concentrations of lactose, lactic acid in cecum and short-chain fatty acids (SCFAs) in cecum and portal vein were measured, meanwhile the expression of ion transporters were ascertained. Our data suggest that the SL, PL and cecal microbiota transplantation (CMT) significantly decreased fecal water content and water intake. In the luminal microbiota there was a significant increase in Akkermansia, Bifidobacterium and Blautia and a lower abundance of Lactobacillus, Escherichia-Shigella, and Dubosiella, while the mucosal microbiota showed a significant increase in Bifidobacterium, Akkermansia, Albaculum, Bilophila, and Coriobacteriaceae_UCG-002 and a lower abundance of Enterococcus, Helicobacter, Dubosiella, and Collinsella. Furthermore, the treatments enhanced lactose fermentation and SCFA production, which may be related to the modulation of the luminal microbial community. A lower ratio of phosphorylation Na/H exchanger3/Na/H exchanger3 (pNHE3/NHE3) and a higher sodium monocarboxylate1 (sMCT1) expression were found in the treatment group than in the model group, which may be related to the changes in the mucosal microbial community. Also, the treatments may restore the impacted metabolic pathways of gut microbiota. These results provide an important foundation for mechanism of SL action and developing PL-based treatment for lactose-induced diarrhea.
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