Molecular detection, isolation and characterization of Peste-des-petits ruminants virus from goat milk from outbreaks in Bangladesh and its implication for eradication strategy.
Molecular detection, isolation and characterization of Peste-des-petits ruminants virus from goat milk from outbreaks in Bangladesh and its implication for eradication strategy.
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DOI：
10.1111/tbed.12911
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发表时间：
2018-12
影响因子：
4.3
通讯作者：
Parida S
中科院分区：
文献类型：
--
作者：
Clarke BD;Islam MR;Yusuf MA;Mahapatra M;Parida S
小反刍兽疫（PPR）是一种高度传染性的小型反刍动物跨界病毒性疾病，在非洲、中东和亚洲大部分地区流行。在南亚，PPR是印度次大陆（包括孟加拉国）的重大关切，因为世界上30%以上的绵羊和山羊在该地区养殖，主要是由小型，贫穷和边缘化的农民。从孟加拉国PPR疫情（2012-2015年）的现场样本中的羊奶中检测并分离出PPR病毒，并获得其全长序列。孟加拉国分离株N基因部分序列分析表明，与邻近的印度病毒相比，其同源性为99.3%-100%，而98.2%-99.6%。对全长基因组的进一步分析表明，孟加拉国分离株之间的同一性为99.3%-99.99%，与邻近的印度病毒的同一性为98.3%-98.4%。这些发现进一步支持了PPR病毒跨越印度和孟加拉国边境的跨境传播。此外，在印度和孟加拉国之间建立跨境战略对于消除该地区的PPR至关重要。从牛奶中分子检测和分离PPR病毒是该疾病传播到自由地区的重要潜在问题，因为全球羊奶的主要生产者是PPR流行国家，特别是印度和孟加拉国以及苏丹。牛奶是一种非侵入性的样本类型，随着2030年根除目标的进展，用于检测PPRV的散装羊奶采样对PPRV的区域监测具有实际意义。
Peste‐des‐petits ruminants (PPR) is a highly contagious transboundary viral disease of small ruminants, which is endemic in much of Africa, the Middle East and Asia. In South Asia, PPR is of significant concern to the Indian subcontinent including Bangladesh as more than 30% of the world's sheep and goats are farmed in this region, predominantly by small, poor and marginal farmers. PPR virus was detected and isolated from goat milk from field samples from PPR outbreaks (2012–2015) in Bangladesh and its full‐length sequences obtained. Sequence analysis of the partial N gene of Bangladesh isolates showed 99.3%–100% identity whereas 98.2%–99.6% identity was observed when compared with neighbouring Indian viruses. Further analysis of the full‐length genomes indicated that the Bangladesh isolates were 99.3%–99.99% identical among themselves and 98.3%–98.4% identical to neighbouring Indian viruses. These findings further support the transboundary transmission of PPR virus across the Indian and Bangladesh border. In additional, the establishment of a cross‐border strategy between India and Bangladesh will be of paramount importance for the eradication of PPR in this region. Molecular detection and isolation of PPR virus from milk is of significant potential concern for spread of the disease to free areas as the major producers of goat milk globally are PPR endemic countries in particular India and Bangladesh, as well as Sudan. Milk is a noninvasive sample type and bulk goat milk sampling for the detection of PPRV would be of practical significance for regional surveillance of PPRV as progress is made towards the targeted 2030 eradication.
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DOI：
10.1128/genomea.01023-14
发表时间：
2014-10-23
期刊：
Genome announcements
影响因子：
--
作者：
Muniraju M;Munir M;Banyard AC;Ayebazibwe C;Wensman J;Zohari S;Berg M;Parthiban AR;Mahapatra M;Libeau G;Batten C;Parida S
通讯作者：
Parida S
影响因子：
3.3
作者：
Dhar, P;Sreenivasa, BP;Bandyopadhyay, SK
通讯作者：
Bandyopadhyay, SK
影响因子：
3.1
作者：
Couacy-Hymann, E;Roger, F;Diallo, A
通讯作者：
Diallo, A
影响因子：
3.3
作者：
Fakri, F.;Embarki, T.;Elharrak, M.
通讯作者：
Elharrak, M.
影响因子：
4.3
作者：
Munir, M.;Zohari, S.;Berg, M.
通讯作者：
Berg, M.