Effect of Locally Delivered Minocycline on the Profile of Subgingival Bacterial Genera in Patients with Periodontitis: A Prospective Pilot Study.
Effect of Locally Delivered Minocycline on the Profile of Subgingival Bacterial Genera in Patients with Periodontitis: A Prospective Pilot Study.
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DOI：
10.3390/biom12050719
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发表时间：
2022-05-18
期刊：
影响因子：
5.5
通讯作者：
Komaki, Motohiro
中科院分区：
文献类型：
--
作者：
Morozumi, Toshiya;Nakayama, Yohei;Shirakawa, Satoshi;Imamura, Kentaro;Nohno, Kaname;Nagano, Takatoshi;Miyazawa, Haruna;Hokari, Takahiro;Takuma, Ryo;Sugihara, Shuntaro;Gomi, Kazuhiro;Saito, Atsushi;Ogata, Yorimasa;Komaki, Motohiro
关键词：
本前瞻性试验研究旨在评价盐酸米诺环素软膏（MO）作为洁治和根面平整（SRP）的辅助药物对龈下菌群的影响。共有59名牙周炎患者接受SRP作为初始牙周治疗。在选定的牙周袋与探测深度（PD）为6-9毫米，网站后，表现出阳性反应的细菌测试，使用免疫层析装置随后与MO（SRP + MO组，n = 25）。在显示阴性反应的部位未进行额外治疗（SRP组，n = 34）。除龈下菌斑采样外，还在基线和初始牙周治疗后4周测量临床参数，包括PD、临床附着水平（CAL）、探诊出血（BOP）、菌斑指数和牙龈指数（GI）。采用末端限制性片段长度多态性分析法对龈下菌群进行分析。相对于基线值，两组中PD-、CAL-、BOP-和GI-采样部位的平均评分在治疗后显著降低（p < 0.01）。内部比较显示SRP + MO组中真杆菌属、细小单胞菌属、丝状菌属、韦荣氏球菌属、梭杆菌属、卟啉单胞菌属、普雷沃氏菌属和未知种属的计数显著降低（p < 0.05）。相互比较表明SRP + MO组中韦荣氏球菌属显著减少（p = 0.01）。SRP和局部MO的联合治疗引起龈下微生物群落的变化：特别是韦荣氏球菌的数量。明显减少。
This prospective pilot study aimed to evaluate the effect of minocycline-HCl ointment (MO), locally delivered as an adjunct to scaling and root planing (SRP), on subgingival microflora. A total of 59 periodontitis patients received SRP as an initial periodontal therapy. In the selected periodontal pockets with probing depths (PD) of 6–9 mm, the sites that exhibited a positive reaction following a bacterial test using an immunochromatographic device were subsequently treated with MO (SRP + MO group, n = 25). No additional treatment was performed at sites showing a negative reaction (SRP group, n = 34). In addition to subgingival plaque sampling, measurement of clinical parameters including PD, clinical attachment level (CAL), bleeding on probing (BOP), plaque index and gingival index (GI) were performed at baseline and 4 weeks after the initial periodontal therapy. The subgingival microflora were assessed by terminal restriction fragment-length polymorphism analysis. Relative to baseline values, the mean scores for PD-, CAL-, BOP-, and GI-sampled sites were significantly decreased post treatment in both groups (p < 0.01). The intra-comparisons showed a significant decrease in the counts of the genera Eubacterium, Parvimonas, Filifactor, Veillonella, Fusobacterium, Porphyromonas, Prevotella, and unknown species in the SRP + MO group (p < 0.05). Inter-comparisons indicated a significant decrease in the genera Veillonella in the SRP + MO group (p = 0.01). Combination therapy of SRP and local MO induced a change in the subgingival microbial community: particularly, the number of Veillonella spp. was markedly reduced.
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影响因子：
3.7
作者：
Jünemann S;Prior K;Szczepanowski R;Harks I;Ehmke B;Goesmann A;Stoye J;Harmsen D
通讯作者：
Harmsen D
影响因子：
4.5
作者：
Butera A;Gallo S;Pascadopoli M;Maiorani C;Milone A;Alovisi M;Scribante A
通讯作者：
Scribante A
影响因子：
2.9
作者：
Kakuta E;Nomura Y;Morozumi T;Nakagawa T;Nakamura T;Noguchi K;Yoshimura A;Hara Y;Fujise O;Nishimura F;Kono T;Umeda M;Fukuda M;Noguchi T;Yoshinari N;Fukaya C;Sekino S;Numabe Y;Sugano N;Ito K;Kobayashi H;Izumi Y;Takai H;Ogata Y;Takano S;Minabe M;Makino-Oi A;Saito A;Abe Y;Sato S;Suzuki F;Takahashi K;Sugaya T;Kawanami M;Hanada N;Takashiba S;Yoshie H
通讯作者：
Yoshie H
影响因子：
2.6
作者：
Kamodyova, Natalia;Minarik, Gabriel;Celec, Peter
通讯作者：
Celec, Peter
影响因子：
2.6
作者：
Miyazawa H;Nakajima T;Horimizu M;Okuda K;Sugita N;Yamazaki K;Li L;Hayashi-Okada Y;Arita T;Nishimoto M;Nishida M;Genco RJ;Yamazaki K
通讯作者：
Yamazaki K