Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.
Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.
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DOI：
10.1371/journal.pone.0015652
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发表时间：
2011-02-10
期刊：
影响因子：
3.7
通讯作者：
Steinhoff G
中科院分区：
文献类型：
--
作者：
Gaebel R;Furlani D;Sorg H;Polchow B;Frank J;Bieback K;Wang W;Klopsch C;Ong LL;Li W;Ma N;Steinhoff G
脐带血(CB)、脂肪组织(AT)和骨髓(BM)来源的人骨髓间充质干细胞(HMSC)治疗心肌梗死(MI)的不同疗效尚不清楚。本研究旨在评价不同来源的人骨髓间充质干细胞的再生潜能，并探讨CD105在心脏再生中的作用。雄性SCID小鼠进行LAD结扎，并接受相应的细胞类型(每只动物400.000只)心肌内注射。梗死后6周，BM和CD105+-CB治疗组与CB和未治疗MI组(MI-C)相比，心导管检查显示显著保留了左心功能。实时定量聚合酶链式反应分析细胞存活率与免疫染色测定毛细血管密度结果一致。此外，与MI-C相比，BM-hMSC可显著减轻心脏重构。在体外低氧条件下，CB-hMSC与BM和CD105纯化的CB来源的hMSC相比，胞外酸化和细胞凋亡率显著增加。我们的研究结果表明，不同来源的hMSC在心脏再生中表现出不同的愈合性能，CD105+hMSC在梗死心脏中表现出良好的存活模式，这意味着更有力地保护了心功能。
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105+-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105+ hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.
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