Novel intrinsic factor Yun maintains female germline stem cell fate through Thickveins.
Novel intrinsic factor Yun maintains female germline stem cell fate through Thickveins.
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DOI：
10.1016/j.stemcr.2022.07.014
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发表时间：
2022-09-13
影响因子：
5.9
通讯作者：
Li, Zhouhua
中科院分区：
文献类型：
--
作者：
Zhao, Hang;Li, Zhengran;Kong, Ruiyan;Shi, Lin;Ma, Rui;Ren, Xuejing;Li, Zhouhua
生殖系干细胞（GSC）对于生物体的繁殖至关重要。必须严格控制GSC的自我更新和分化，以避免干细胞增殖失控或干细胞过早分化。然而，GSC的自我更新和分化是如何被适当控制的还没有完全了解。在这里，我们发现，新的内在因子云所需的女性GSC维护果蝇。GSC由于在不存在yun的情况下通过缺陷性BMP/Dpp信号传导对分化因子Bam的去抑制而经历早熟分化。从机制上讲，Yun与Dpp/BMP信号传导的I型受体Thickveins（THEX）相关联并使其稳定。最后，异位表达的组成型活性TkvQD（TkvQD）完全抑制GSC的损失所造成的yun耗尽。总的来说，这些数据表明Yun通过TdR发挥功能以维持GSC命运。我们的研究结果为如何正确控制干细胞维持的调控机制提供了新的见解。新的内在因子Yun是女性GSC维持所必需的Yun缺陷的GSC由于Bam上调而进行分化Yun与Tclad结合并稳定Tclad以调节GSC维持在缺乏Yun的情况下GSC损失可以通过组成型活性Tclad来挽救在这篇文章中，Zhouhua Li和同事表明Yun通过Thickveins（Tclad）维持女性生殖系干细胞（GSC）命运。由于BMP信号传导缺陷和Bam水平增加，种系中yun的消耗导致GSC维持缺陷。Yun结合并稳定TcR，以调节生理条件下GSC的维持。
Germline stem cells (GSCs) are critical for the reproduction of an organism. The self-renewal and differentiation of GSCs must be tightly controlled to avoid uncontrolled stem cell proliferation or premature stem cell differentiation. However, how the self-renewal and differentiation of GSCs are properly controlled is not fully understood. Here, we find that the novel intrinsic factor Yun is required for female GSC maintenance in Drosophila. GSCs undergo precocious differentiation due to de-repression of differentiation factor Bam by defective BMP/Dpp signaling in the absence of yun. Mechanistically, Yun associates with and stabilizes Thickveins (Tkv), the type I receptor of Dpp/BMP signaling. Finally, ectopic expression of a constitutively active Tkv (TkvQD) completely suppresses GSC loss caused by yun depletion. Collectively, these data demonstrate that Yun functions through Tkv to maintain GSC fate. Our results provide new insight into the regulatory mechanisms of how stem cell maintenance is properly controlled. Novel intrinsic factor Yun is required for female GSC maintenance Yun-defective GSCs undergo differentiation due to Bam upregulation Yun associates with and stabilizes Tkv to regulate GSC maintenance GSC loss in the absence of yun could be rescued by constitutively active Tkv In this article, Zhouhua Li and colleagues show that Yun maintains female germline stem cell (GSC) fate through Thickveins (Tkv). Depletion of yun in the germline causes GSC maintenance defects due to defective BMP signaling and increased Bam levels. Yun associates with and stabilizes Tkv to regulate GSC maintenance under physiological conditions.
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影响因子：
10.5
作者：
MCKEARIN, DM;SPRADLING, AC
通讯作者：
SPRADLING, AC
影响因子：
8.5
作者：
通讯作者：
--
DOI：
10.1083/jcb.200712159
发表时间：
2008-01-28
期刊：
The Journal of cell biology
影响因子：
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通讯作者：
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作者：
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11.8
作者：
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通讯作者：
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