Prevalence and characterization of mecC MRSA in bovine bulk tank milk in Great Britain, 2017-18.
Prevalence and characterization of mecC MRSA in bovine bulk tank milk in Great Britain, 2017-18.
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2017-18年英国牛散装罐乳中mecC MRSA的流行率和特征。
DOI：
10.1093/jacamr/dlaa125
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发表时间：
2021-03
影响因子：
3.4
通讯作者：
Holmes MA
中科院分区：
文献类型：
--
作者：
Cui C;Ba X;Holmes MA
为了评估在英格兰和威尔士的奶牛场中mecC MRSA的当前流行状况，在2011-12年进行了上一次调查后的5 年。在2017-18年进行的这项研究中使用了英格兰和威尔士697个奶场的便利样本，使用高盐肉汤浓缩和显色MRSA琼脂选择来检测散装罐装牛奶样本中mecC MRSA的存在。所有可能的MRSA分离株均通过聚合酶链式反应筛选mecA和mecC基因，并用纸片扩散法和VITEK®2进行药敏试验。在英格兰和威尔士的697个奶牛场中，有4个奶牛场检出MECC MRSA(患病率为0.57%，95%CI为0.16%-1.46%)。其中3株为ST425，1株为ST4652(CC130谱系)。同时还分离到两株mecA MRSA：一株为ST5，一株为ST398。这些结果表明，与之前的一项研究相比，英格兰和威尔士的mecC MRSA患病率大幅下降，下降了72%(2.15%至0.57%)。虽然mecA MRSA的水平仍然很低，但与牲畜相关的MRSA ST398的持续存在表明，这种血统在英国已经确立。
To evaluate the current prevalence status of mecC MRSA among dairy farms in England and Wales 5 years after a previous survey conducted in 2011–12. A convenience sample of 697 dairy farms in England and Wales was used for the study, conducted in 2017–18, testing bulk tank milk samples for the presence of mecC MRSA using high salt broth enrichment and chromogenic MRSA agar selection. All putative MRSA isolates were screened by PCR for the presence of mecA and mecC genes and subjected to antimicrobial susceptibility testing using both the disc diffusion method and VITEK® 2. MRSA isolates were also sequenced for genomic characterization. mecC MRSA were detected on 4 out of 697 dairy farms in England and Wales (prevalence 0.57%, 95% CI 0.16%–1.46%). Three of the mecC isolates were ST425 and one was ST4652 (in the CC130 lineage). Two mecA MRSA were also isolated: one ST5 and one ST398. These results indicate that there has been a substantial reduction in the prevalence of mecC MRSA in England and Wales with a 72% reduction (2.15% to 0.57%) compared with a previous study. While the levels of mecA MRSA remain very low the continued presence of ST398, a livestock-associated MRSA, suggests that this lineage is established in the UK.
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影响因子：
3.7
作者：
Stegger M;Liu CM;Larsen J;Soldanova K;Aziz M;Contente-Cuomo T;Petersen A;Vandendriessche S;Jiménez JN;Mammina C;van Belkum A;Salmenlinna S;Laurent F;Skov RL;Larsen AR;Andersen PS;Price LB
通讯作者：
Price LB
影响因子：
56.3
作者：
Garcia-Alvarez, Laura;Holden, Matthew T. G.;Lindsay, Heather;Webb, Cerian R.;Brown, Derek F. J.;Curran, Martin D.;Walpole, Enid;Brooks, Karen;Pickard, Derek J.;Teale, Christopher;Parkhill, Julian;Bentley, Stephen D.;Edwards, Giles F.;Girvan, E. Kirsty;Kearns, Angela M.;Pichon, Bruno;Hill, Robert L. R.;Larsen, Anders Rhod;Skov, Robert L.;Peacock, Sharon J.;Maskell, Duncan J.;Holmes, Mark A.
通讯作者：
Holmes, Mark A.
影响因子：
12.3
作者：
Tonkin-Hill, Gerry;MacAlasdair, Neil;Parkhill, Julian
通讯作者：
Parkhill, Julian
DOI：
10.1093/jac/dkt462
发表时间：
2014-04
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Paterson GK;Morgan FJ;Harrison EM;Cartwright EJ;Török ME;Zadoks RN;Parkhill J;Peacock SJ;Holmes MA
通讯作者：
Holmes MA
影响因子：
9.4
作者：
Becker, Karsten;Larsen, Anders R.;Kriegeskorte, Andre
通讯作者：
Kriegeskorte, Andre