Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules.
Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules.
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DOI：
10.1083/jcb.152.5.959
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发表时间：
2001-03-05
期刊：
影响因子：
--
通讯作者：
Margolis B
中科院分区：
文献类型：
--
作者：
Verhey KJ;Meyer D;Deehan R;Blenis J;Schnapp BJ;Rapoport TA;Margolis B
分子马达驱动蛋白沿着沿着微管移动的货物一直是难以捉摸的。我们寻找驱动蛋白轻链羧基端的结合伴侣，它含有tetratricopeptide repeat（TPR）基序。发现了三种蛋白质，即c-jun NH 2末端激酶（JNK）相互作用蛋白（JIP）JIP-1、JIP-2和JIP-3，它们是JNK信号通路的支架蛋白。神经末梢中JIP的浓度需要驱动蛋白，从JIP COOH末端突变体和显性负驱动蛋白构建体的分析中可以看出。共沉淀实验表明，驱动蛋白携带的JIP支架预装细胞质（双亮氨酸拉链轴承激酶）和跨膜信号分子（Reelin受体，ApoER 2）。这些结果表明，传统的驱动蛋白和货物之间的直接相互作用，表明马达蛋白通过支架蛋白连接到其膜货物，并支持马达蛋白在信号转导途径的空间调节中的作用。
The cargo that the molecular motor kinesin moves along microtubules has been elusive. We searched for binding partners of the COOH terminus of kinesin light chain, which contains tetratricopeptide repeat (TPR) motifs. Three proteins were found, the c-jun NH2-terminal kinase (JNK)–interacting proteins (JIPs) JIP-1, JIP-2, and JIP-3, which are scaffolding proteins for the JNK signaling pathway. Concentration of JIPs in nerve terminals requires kinesin, as evident from the analysis of JIP COOH-terminal mutants and dominant negative kinesin constructs. Coprecipitation experiments suggest that kinesin carries the JIP scaffolds preloaded with cytoplasmic (dual leucine zipper–bearing kinase) and transmembrane signaling molecules (the Reelin receptor, ApoER2). These results demonstrate a direct interaction between conventional kinesin and a cargo, indicate that motor proteins are linked to their membranous cargo via scaffolding proteins, and support a role for motor proteins in spatial regulation of signal transduction pathways.
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DOI：
10.1083/jcb.150.2.f63
发表时间：
2000-07-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goldstein LS;Gunawardena S
通讯作者：
Gunawardena S
影响因子：
56.9
作者：
GHO, M;MCDONALD, K;SAXTON, WM
通讯作者：
SAXTON, WM
影响因子：
56.9
作者：
Brendza, RP;Serbus, LR;Saxton, WM
通讯作者：
Saxton, WM
影响因子：
21.3
作者：
Coy, DL;Hancock, WO;Howard, J
通讯作者：
Howard, J
影响因子：
64.5
作者：
Bowman, AB;Kamal, A;Goldstein, LSB
通讯作者：
Goldstein, LSB