Quantitative analysis of the excitability of hypoglossal motoneurons during natural sleep in the rat.
Quantitative analysis of the excitability of hypoglossal motoneurons during natural sleep in the rat.
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DOI：
10.1016/j.jneumeth.2012.09.009
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发表时间：
2013-01-15
影响因子：
3
通讯作者：
Chase, Michael H.
中科院分区：
文献类型：
--
作者：
Fenik, Victor B.;Fung, Simon J.;Lim, Vincent;Chase, Michael H.
我们描述了一种新的方法来评估大鼠在自然发生的睡眠和觉醒状态下舌下神经运动神经元的兴奋性。成年大鼠手术制备永久放置的电极记录脑电图，眼电图和颈部肌电图。在完整的舌下神经周围植入刺激/记录微型三极袖状电极，并将头部约束装置粘合到颅骨。在对头部约束装置适应一段时间后，动物没有表现出任何不适迹象，并且很容易在觉醒、NREM和REM睡眠状态之间转换。在睡眠或清醒时，舌下神经没有自发呼吸或紧张活动。舌下神经运动神经元被激活的舌下神经的电刺激（逆向）或微刺激直接施加到舌下神经核。微刺激舌下神经运动神经元诱发同侧舌下神经复合动作电位。与NREM睡眠期间的积分幅度相比，清醒期间的积分幅度往往较高（112.6% ± 15;标准差），REM睡眠期间的积分幅度强烈降低（24.7% ± 3.4）。采用压力显微注射法将利多卡因注入微刺激部位，证实了舌下神经诱发的反应可以受到间接影响。我们的结论是，这种动物模型可以用来研究神经递质的机制，控制舌下神经运动神经元的兴奋性，在自然发生的睡眠和觉醒状态。
We describe a novel approach to assess the excitability of hypoglossal motoneurons in rats during naturally-occurring states of sleep and wakefulness. Adult rats were surgically prepared with permanently-placed electrodes to record the EEG, EOG and neck EMG. A stimulating/recording miniature tripolar cuff electrode was implanted around the intact hypoglossal nerve and a head-restraining device was bonded to the calvarium. After a period of adaptation to head-restraint, the animals did not exhibit any sign of discomfort and readily transitioned between the states of wakefulness, NREM and REM sleep. There was no spontaneous respiratory or tonic activity present in the hypoglossal nerve during sleep or wakefulness. Hypoglossal motoneurons were activated by electrical stimulation of the hypoglossal nerve (antidromically) or by microstimulation directly applied to the hypoglossal nucleus. Microstimulation of hypoglossal motoneurons evoked compound action potentials in the ipsilateral hypoglossal nerve. The magnitude of their integrals tended to be higher during wakefulness (112.6% ± 15; standard deviation) and were strongly depressed during REM sleep (24.7% ± 3.4), compared to the integral magnitude during NREM sleep. Lidocaine, which was delivered using pressure microinjection to the microstimulation site, verified that the responses evoked in hypoglossal nerve can be affected pharmacologically. We conclude that this animal model can be utilized to study the neurotransmitter mechanisms that control the excitability of hypoglossal motoneurons during naturally-occurring states of sleep and wakefulness.
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影响因子：
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