Superresolution optical fluctuation imaging with organic dyes.
Superresolution optical fluctuation imaging with organic dyes.
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DOI：
10.1002/anie.201004138
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发表时间：
2010-12-03
影响因子：
16.6
通讯作者：
Weiss, Shimon
中科院分区：
文献类型：
--
作者：
Dertinger, Thomas;Heilemann, Mike;Vogel, Robert;Sauer, Markus;Weiss, Shimon
超分辨率远场显微镜自1994年第一个概念提出以来，经历了巨大的发展，扩大了荧光显微镜的范围和应用，超出了衍射极限。各种强大而优秀的方法，如受激发射耗尽[2]，饱和结构照明显微镜[3]和随机单分子开关方法，如STORM [4]，(f) PALM [5,6], directSTORM (dSTORM)[7,8]和GSDIM[9]。值得注意的是，所有超分辨率方法的关键在于利用分子的两态转变（例如荧光“开”和非荧光“关”状态）。特别是对于随机单分子开关方法（PALM， STORM等），需要精确控制荧光团在荧光状态和暗状态下花费的时间。在dSTORM概念中，这是通过在还原剂存在的情况下用一个或两个波长照射样品来实现的。同时，还原性和氧化性缓冲系统（ROXS）的发明使得大多数有机染料的荧光间歇率几乎可调到任何程度[10,11]。直接后果是
Superresolution far-field microscopy has experienced a tremendous growth since the introduction of the first concept in 1994 [1], broadening the scope and applications of fluorescence microscopy beyond the diffraction limit. Various powerful and excellent methods, such as stimulated emission depletion [2], saturated structured illumination microscopy [3] and stochastic single-molecule switching methods such as STORM [4],(f) PALM [5, 6], directSTORM (dSTORM)[7, 8] and GSDIM [9], have been developed since. Notably, the key to all superresolution methods lies in the exploitation of a two-state transition (eg a fluorescent ‘on’and a non-fluorescent ‘off’state) of the molecules. Especially for the stochastic single-molecule switching methods (PALM, STORM etc.) it is necessary to precisely control both the time a fluorophore spends in the fluorescent as well as in the dark state. In the dSTORM concept, this is achieved by irradiation of the sample with one or two wavelengths in the presence of reducing agents. In parallel, the invention of a reducing and oxidizing buffer system (ROXS) made the fluorescence intermittency rates of most organic dyes tuneable to almost any degree [10, 11]. Immediate consequences are
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影响因子：
15
作者：
Steinhauer, Christian;Forthmann, Carsten;Tinnefeld, Philip
通讯作者：
Tinnefeld, Philip
影响因子：
3.8
作者：
Dertinger T;Colyer R;Vogel R;Enderlein J;Weiss S
通讯作者：
Weiss S
DOI：
10.1073/pnas.0907866106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Dertinger, T.;Colyer, R.;Enderlein, J.
通讯作者：
Enderlein, J.
影响因子：
13.3
作者：
Kasper, Robert;Harke, Benjamin;Sauer, Markus
通讯作者：
Sauer, Markus
影响因子：
46.9
作者：
Hell, SW
通讯作者：
Hell, SW