Lactobacillus-mediated priming of the respiratory mucosa protects against lethal pneumovirus infection.
Lactobacillus-mediated priming of the respiratory mucosa protects against lethal pneumovirus infection.
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DOI：
10.4049/jimmunol.1001751
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发表时间：
2011-01-15
期刊：
影响因子：
--
通讯作者：
Rosenberg HF
中科院分区：
文献类型：
--
作者：
Gabryszewski SJ;Bachar O;Dyer KD;Percopo CM;Killoran KE;Domachowske JB;Rosenberg HF
对呼吸道病毒感染的炎症反应可能是复杂的，并且对标准治疗是难治的。乳酸杆菌，当靶向呼吸道上皮细胞时，在抑制病毒诱导的炎症和防止致命疾病方面非常有效。具体而言，通过鼻内接种活的或热灭活的植物乳杆菌或罗伊氏乳杆菌致敏的野生型小鼠完全免受致命的啮齿动物病原体小鼠肺炎病毒（PVM）的致命感染;显著的保护（60%存活率）持续至少13周。保护不是乳杆菌属物种所独有的，并且也在响应于用非致病性革兰氏阳性无害李斯特菌引发时观察到。用活乳酸杆菌引发导致粒细胞募集减少，多种促炎细胞因子（CXCL 10，CXCL 1，CCL 2和TNF）表达减少，病毒回收减少，尽管我们已经清楚地证明绝对病毒滴度不能预测临床结果。乳酸杆菌引发还导致延长存活时间，并保护MyD 88基因缺失（MyD 88 −/−）小鼠免受PVM感染的致命后遗症，这表明保护机制可能是Toll样受体无关的。最有趣的是，乳酸杆菌致敏的MyD 88 −/−小鼠中的病毒恢复和细胞因子表达模式与对照致敏的MyD 88 −/−小鼠中观察到的病毒恢复和细胞因子表达模式无法区分，总之，我们已经确定并表征了一种有效的乳酸杆菌介导的先天免疫屏障，该屏障最终可能在缺乏特异性抗病毒疫苗的情况下对感染起到关键和长期的保护作用。
The inflammatory response to respiratory virus infection can be complex and refractory to standard therapy. Lactobacilli, when targeted to the respiratory epithelium, are highly effective at suppressing virus-induced inflammation and protecting against lethal disease. Specifically, wild-type mice primed via intranasal inoculation with live or heat-inactivated Lactobacillus plantarum or Lactobacillus reuteri were completely protected against lethal infection with the virulent rodent pathogen, pneumonia virus of mice (PVM); significant protection (60% survival) persisted for at least thirteen weeks. Protection was not unique to Lactobacillus species, and was also observed in response to priming with non-pathogenic gram-positive Listeria innocua. Priming with live lactobacilli resulted in diminished granulocyte recruitment, diminished expression of multiple proinflammatory cytokines (CXCL10, CXCL1, CCL2, and TNF) and reduced virus recovery, although we have demonstrated clearly that absolute virus titer does not predict clinical outcome. Lactobacillus priming also resulted in prolonged survival and protection against the lethal sequelae of PVM infection in MyD88 gene-deleted (MyD88−/−) mice, suggesting that the protective mechanisms may be Toll-like receptor-independent. Most intriguing, virus recovery and cytokine expression patterns in Lactobacillus-primed MyD88−/− mice were indistinguishable from those observed in control-primed MyD88−/− counterparts, In summary, we have identified and characterized an effective Lactobacillus-mediated innate immune shield, which may ultimately serve as critical and long-term protection against infection in the absence of specific antiviral vaccines.
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影响因子：
5.4
作者：
Bonville, CA;Easton, AJ;Domachowske, JB
通讯作者：
Domachowske, JB
DOI：
10.1056/nejmoa0804877
发表时间：
2009-02-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hall CB;Weinberg GA;Iwane MK;Blumkin AK;Edwards KM;Staat MA;Auinger P;Griffin MR;Poehling KA;Erdman D;Grijalva CG;Zhu Y;Szilagyi P
通讯作者：
Szilagyi P
DOI：
10.1099/00221287-143-8-2733
发表时间：
1997-08-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Hols, P;Slos, P;Mercenier, A
通讯作者：
Mercenier, A
影响因子：
15.3
作者：
Didierlaurent, Arnaud;Goulding, John;Patel, Seema;Snelgrove, Robert;Low, Lionel;Bebien, Magali;Lawrence, Toby;van Rijt, Leonie S.;Lambrecht, Bart N.;Sirard, Jean-Claude;Hussell, Tracy
通讯作者：
Hussell, Tracy
影响因子：
2.4
作者：
Harata, G.;He, F.;Yausi, H.
通讯作者：
Yausi, H.