Differences in Glycolysis and Mitochondrial Respiration between Cytotrophoblast and Syncytiotrophoblast In-Vitro: Evidence for Sexual Dimorphism.
Differences in Glycolysis and Mitochondrial Respiration between Cytotrophoblast and Syncytiotrophoblast In-Vitro: Evidence for Sexual Dimorphism.
复制标题
DOI：
10.3390/ijms221910875
复制
发表时间：
2021-10-08
影响因子：
5.6
通讯作者：
Myatt L
中科院分区：
文献类型：
--
作者：
Bucher M;Kadam L;Ahuna K;Myatt L
在胎盘中，增殖的细胞滋养层细胞融合成终末分化的合体滋养层，合体滋养层承担多种能量密集型功能，包括营养摄取和转移以及激素合成。我们使用海马糖酵解和线粒体压力测试的滋养层细胞分离的健康体重的妇女在任期内，以评估细胞滋养层（CT）和合体滋养层（ST）有不同的生物能量的战略，鉴于其不同的功能。而在基础糖酵解没有差异，CT有显着更大的糖酵解能力和储备比ST。相反，ST有显着更高的基础，ATP偶联和最大线粒体呼吸和备用容量比CT。因此，在应激条件下，CT可以通过其更高的糖酵解能力增加能量产生，而ST可以使用其更高和更有效的线粒体呼吸能力。我们以前已经表明，在子宫内的糖尿病和肥胖症的不利条件下，滋养层呼吸是两性异形的。我们发现性别之间的糖酵解参数没有差异，线粒体呼吸参数没有差异，但在合体化后观察到的增加似乎在女性中更大。代谢灵活性存在差异，即，利用葡萄糖、谷氨酰胺或脂肪酸的能力，在两性之间的合胞体化中观察到，雌性滋养层的灵活性增加，表明适应营养供应变化的能力更好。
In the placenta the proliferative cytotrophoblast cells fuse into the terminally differentiated syncytiotrophoblast layer which undertakes several energy-intensive functions including nutrient uptake and transfer and hormone synthesis. We used Seahorse glycolytic and mitochondrial stress tests on trophoblast cells isolated at term from women of healthy weight to evaluate if cytotrophoblast (CT) and syncytiotrophoblast (ST) have different bioenergetic strategies, given their different functions. Whereas there are no differences in basal glycolysis, CT have significantly greater glycolytic capacity and reserve than ST. In contrast, ST have significantly higher basal, ATP-coupled and maximal mitochondrial respiration and spare capacity than CT. Consequently, under stress conditions CT can increase energy generation via its higher glycolytic capacity whereas ST can use its higher and more efficient mitochondrial respiration capacity. We have previously shown that with adverse in utero conditions of diabetes and obesity trophoblast respiration is sexually dimorphic. We found no differences in glycolytic parameters between sexes and no difference in mitochondrial respiration parameters other than increases seen upon syncytialization appear to be greater in females. There were differences in metabolic flexibility, i.e., the ability to use glucose, glutamine, or fatty acids, seen upon syncytialization between the sexes with increased flexibility in female trophoblast suggesting a better ability to adapt to changes in nutrient supply.
登录
查看更多内容
影响因子：
7.4
作者：
Dranka, Brian P.;Hill, Bradford G.;Darley-Usmar, Victor M.
通讯作者：
Darley-Usmar, Victor M.
影响因子：
3.8
作者：
EIS, ALW;BROCKMAN, DE;MYATT, L
通讯作者：
MYATT, L
DOI：
10.1073/pnas.1000440107
发表时间：
2010-03-23
影响因子：
11.1
作者：
Mao, Jiude;Zhang, Xia;Rosenfeld, Cheryl S.
通讯作者：
Rosenfeld, Cheryl S.
影响因子：
64.5
作者：
Faubert B;Li KY;Cai L;Hensley CT;Kim J;Zacharias LG;Yang C;Do QN;Doucette S;Burguete D;Li H;Huet G;Yuan Q;Wigal T;Butt Y;Ni M;Torrealba J;Oliver D;Lenkinski RE;Malloy CR;Wachsmann JW;Young JD;Kernstine K;DeBerardinis RJ
通讯作者：
DeBerardinis RJ
影响因子：
5.5
作者：
Larsen, Steen;Nielsen, Joachim;Hey-Mogensen, Martin
通讯作者：
Hey-Mogensen, Martin