P-type Na(+)/K(+) ATPases essential and nonessential for cellular homeostasis and insect pathogenicity of Beauveria bassiana.
P-type Na(+)/K(+) ATPases essential and nonessential for cellular homeostasis and insect pathogenicity of Beauveria bassiana.
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白僵菌细胞稳态和昆虫致病性必需和非必需的 P 型 Na /K ATP 酶
DOI：
10.1080/21505594.2020.1836903
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发表时间：
2020-12
期刊：
影响因子：
5.2
通讯作者：
Feng MG
中科院分区：
文献类型：
--
作者：
Mou YN;Gao BJ;Ren K;Tong SM;Ying SH;Feng MG
ENA 1和ENA 2是酵母细胞内稳态所需的P型IID/ENA Na+/K+-ATP酶，但在丝状真菌昆虫病原体中仍知之甚少。在这里，我们确定了7个基因编码的5个ENA 1/2同源物（ENA 1a-c和ENA 2a/B）和两个P-型IIC/NK Na+/K+-ATP酶（NK 1/2）的球孢白僵菌，一种昆虫病原真菌作为全球真菌杀虫剂的主要来源。这些基因中的大多数在转录水平上对碱性pH和Na+/K+信号高度敏感。细胞Na+，K+和H+稳态被打乱，只有在ena 1a或ena 2b的情况下。在5天的浸没培养期间，受干扰的内稳态的特征是液泡酸化加速，细胞溶质Na+/K+水平在pH 5.0至9.0升高，细胞外H+水平稳定至初始pH 7.5。突变体Δ ena 1a和Δ ena 2b对金属离子（Na+、K+、Li+、Zn 2+、Mn 2+和Fe 3+）、细胞壁扰动、氧化、非阳离子高渗和UVB辐射的耐受性较差，通过表皮感染引起的昆虫致病性严重降低，通过血腔注射引起的毒力减弱。其他5个ENA和NK基因的缺失突变体在液泡pH和所有检查的表型中表现出很小的变化。因此，只有ENA 1 a和ENA 2 B b明显参与跨膜和液泡活动，是B细胞阳离子平衡、昆虫致病性和多重胁迫耐受性所必需的。巴西纳。这些发现为研究ENA 1a和ENA 2b依赖的液泡pH稳定性、阳离子稳态过程以及真菌对宿主昆虫和环境的适应性提供了新的视角。
ENA1 and ENA2 are P-type IID/ENA Na+/K+-ATPases required for cellular homeostasis in yeasts but remain poorly understood in filamentous fungal insect pathogens. Here, we characterized seven genes encoding five ENA1/2 homologues (ENA1a–c and ENA2a/b) and two P-type IIC/NK Na+/K+-ATPases (NK1/2) in Beauveria bassiana, an insect-pathogenic fungus serving as a main source of fungal insecticides worldwide. Most of these genes were highly responsive to alkaline pH and Na+/K+ cues at transcription level. Cellular Na+, K+ and H+ homeostasis was disturbed only in the absence of ena1a or ena2b. The disturbed homeostasis featured acceleration of vacuolar acidification, elevation of cytosolic Na+/K+ level at pH 5.0 to 9.0, and stabilization of extracellular H+ level to initial pH 7.5 during a 5-day period of submerged incubation. Despite little defect in hyphal growth and asexual development, the Δena1a and Δena2b mutants were less tolerant to metal cations (Na+, K+, Li+, Zn2+, Mn2+ and Fe3+), cell wall perturbation, oxidation, non-cation hyperosmolarity and UVB irradiation, severely compromised in insect pathogenicity via normal cuticle infection, and attenuated in virulence via hemocoel injection. The deletion mutants of five other ENA and NK genes showed little change in vacuolar pH and all examined phenotypes. Therefore, only ENA1a and ENA2b evidently involved in both transmembrane and vacuolar activities are essential for cellular cation homeostasis, insect pathogenicity and multiple stress tolerance in B. bassiana. These findings provide a novel insight into ENA1a- and ENA2b-dependent vacuolar pH stability, cation-homeostatic process and fungal fitness to host insect and environment.
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五种液泡 Ca2 交换器在钙调神经磷酸酶依赖性 Ca2/Mn2 耐受性、多重应激反应和丝状昆虫病原体的毒力中发挥不同的作用
影响因子：
3
作者：
Hu, Yue;Wang, Jie;Feng, Ming-Guang
通讯作者：
Feng, Ming-Guang
影响因子：
4.5
作者：
Gao Q;Jin K;Ying SH;Zhang Y;Xiao G;Shang Y;Duan Z;Hu X;Xie XQ;Zhou G;Peng G;Luo Z;Huang W;Wang B;Fang W;Wang S;Zhong Y;Ma LJ;St Leger RJ;Zhao GP;Pei Y;Feng MG;Xia Y;Wang C
通讯作者：
Wang C
影响因子：
3.2
作者：
Almagro, A;Prista, C;Ramos, J
通讯作者：
Ramos, J
影响因子：
3.3
作者：
Shang Y;Xiao G;Zheng P;Cen K;Zhan S;Wang C
通讯作者：
Wang C
影响因子：
3.2
作者：
Krauke, Yannick;Sychrova, Hana
通讯作者：
Sychrova, Hana