Population genetic diversity and dynamics of the honey bee brood pathogen Melissococcus plutonius in a region with high prevalence.
Population genetic diversity and dynamics of the honey bee brood pathogen Melissococcus plutonius in a region with high prevalence.
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DOI：
10.1016/j.jip.2022.107867
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发表时间：
2023-02
影响因子：
3.4
通讯作者：
Dietemann, Vincent
中科院分区：
文献类型：
--
作者：
Grossar, Daniela;Haynes, Edward;Budge, Giles E.;Parejo, Melanie;Gauthier, Laurent;Charriere, Jean-Daniel;Chapuisat, Michel;Dietemann, Vincent
蜜蜂病原菌M.冥王星。鉴定了5个新M.冥王星层序类型推定毒力因子蜂毒毒素A的出现时间增加。欧洲蜜蜂幼虫病（European foulbrood，EFB）是一种由细菌Melissococcus plutonius引起的蜜蜂幼虫病。近几十年来，一些国家报告了大规模的EFB爆发，这需要对受影响的养蜂场采取昂贵的卫生措施，以限制这种传染性病原体的传播。为了减轻其影响，需要更好地了解病原体的种群动态。本研究采用多位点序列分型（MLST）技术对160株M的遗传多样性和地理分布进行了初步研究。plutonius分离物收集自EFB症状的蜜蜂群，相隔7年。分离株属于3个克隆复合体（CC），全球已知的12个序列类型（ST），其中5个是新的。系统发育和聚类分析表明，这些新的序列类型中的一些可能在爆发期间局部进化，但大多数又消失了。我们进一步筛选蜂毒毒素A（mtxA），一个假定的毒力基因的菌株。随着时间的推移，mtxA频繁出现的ST的患病率增加，这表明该基因促进了传播。尽管该基因在人群中的频率增加，但病例总数减少，这可能是由于在第二个采样期之前实施了更严格的控制措施。我们的结果提供了一个更好的理解M。plutonius种群动态并帮助确定限制有效控制这种新兴疾病的知识差距。
MLST and spatial analysis of the honey bee pathogen M. plutonius. Identification of five novel M. plutonius sequence types. Temporal increase in the occurrence of the putative virulence factor melissotoxin A. European foulbrood (EFB) is a honey bee brood disease caused by the bacterium Melissococcus plutonius. Large-scale EFB outbreaks have been reported in several countries in recent decades, which entail costly sanitation measures of affected apiaries to restrict the spread of this contagious pathogen. To mitigate its impact, a better understanding of the population dynamics of the etiological agent is required. We here used multi-locus sequence typing (MLST) to infer the genetic diversity and geographical distribution of 160 M. plutonius isolates collected from EFB symptomatic honey bee colonies seven years apart. Isolates belonged to three clonal complexes (CCs) known worldwide and to 12 sequence types (STs), of which five were novel. Phylogenetic and clustering analyses showed that some of these novel sequence types have likely evolved locally during a period of outbreak, but most disappeared again. We further screened the isolates for melissotoxin A (mtxA), a putative virulence gene. The prevalence of STs in which mtxA was frequent increased over time, suggesting that this gene promotes spread. Despite the increased frequency of this gene in the population, the total number of cases decreased, which could be due to stricter control measures implemented before the second sampling period. Our results provide a better understanding of M. plutonius population dynamics and help identify knowledge gaps that limit efficient control of this emerging disease.
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影响因子：
1.9
作者：
Dahle, Bjorn
通讯作者：
Dahle, Bjorn
影响因子：
3.5
作者：
Djukic M;Erler S;Leimbach A;Grossar D;Charrière JD;Gauthier L;Hartken D;Dietrich S;Nacke H;Daniel R;Poehlein A
通讯作者：
Poehlein A
影响因子：
3.7
作者：
Cornman RS;Tarpy DR;Chen Y;Jeffreys L;Lopez D;Pettis JS;vanEngelsdorp D;Evans JD
通讯作者：
Evans JD
DOI：
10.1038/ismej.2014.20
发表时间：
2014-08
期刊：
The ISME journal
影响因子：
--
作者：
Budge GE;Shirley MD;Jones B;Quill E;Tomkies V;Feil EJ;Brown MA;Haynes EG
通讯作者：
Haynes EG
DOI：
10.1080/0005772x.2005.11417323
发表时间：
2005-12-01
期刊：
BEE WORLD
影响因子：
--
作者：
Ellis, JD;Munn, PA
通讯作者：
Munn, PA