Sex-related differences in pattern of ethanol drinking under the intermittent-access model and its impact on exploratory and anxiety-like behavior in Long-Evans rats.
Sex-related differences in pattern of ethanol drinking under the intermittent-access model and its impact on exploratory and anxiety-like behavior in Long-Evans rats.
复制标题
在Long-Evans大鼠中，无意识获取模型下乙醇饮用模式的性别相关差异及其对探索和焦虑样行为的影响。
DOI：
10.1111/acer.14853
复制
发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Barson, Jessica R.
中科院分区：
文献类型：
--
作者：
Pirino, Breanne E.;Martin, Cydney R.;Carpenter, Brody A.;Curtis, Genevieve R.;Curran-Alfaro, Christina M.;Samels, Shanna B.;Barker, Jacqueline M.;Karkhanis, Anushree N.;Barson, Jessica R.
虽然美国男性比女性饮酒更多，但饮酒率正在趋同。尽管如此，女性在临床前酒精研究中的代表性仍然不足。在这里，我们检查了大鼠乙醇饮用模式中与性别相关的差异，以及这种饮酒对探索和焦虑样行为的影响。成年雄性和雌性 Long-Evans 大鼠在间歇性两瓶选择模式下给予 20% 乙醇。前 7 周每天测量他们的摄入量。在第 8 周期间，测量每日 24 小时内的摄入量。在第 9 周，他们与未接触乙醇的对照组一起在每天进入新颖的室、明暗盒和孔板装置中进行测试。在第 10 周期间，在访问 30 – 40 分钟后评估血液乙醇浓度 (BEC)。与男性相比，女性在所有访问周内总体表现出更高的乙醇摄入量和偏好，尽管只有一半的女性饮酒量明显多于男性。在每天 24 小时的访问中，两性在前 30 分钟的摄入量最高，在明/暗周期的光照阶段的中间摄入量最低。尽管女性的乙醇摄入量较多，但 BEC 与男性相比并没有显着差异。在行为测试中，与男性相比，女性在新型活动室中的垂直时间较短，在明暗盒中的房间之间的运动较多，在孔板装置中的鼻子戳更多。虽然饮用乙醇的历史导致了活动室中垂直时间减少和明暗箱中的室进入量增加的趋势，但这与性别无关。这些结果表明，雌性和雄性大鼠每天摄入 30 分钟后都可以测试乙醇的急性影响，但在设计这些实验和解释结果时应仔细考虑。
While men in the United States consume more alcohol than women, rates of drinking are converging. Nevertheless, females remain underrepresented in preclinical alcohol research. Here, we examined rats for sex-related differences in patterns of ethanol drinking and effects of this drinking on exploratory and anxiety-like behavior. Adult male and female Long-Evans rats were given 20% ethanol under the intermittent-access two-bottle-choice paradigm. Their intake was measured daily for the first 7 weeks. During the 8th week, intake was measured over the 24 hours of daily access. During the 9th week, they, along with ethanol-naive controls, were tested prior to daily access in a novel chamber, light-dark box, and hole board apparatus. During the 10th week, blood ethanol concentration (BEC) was assessed after 30 – 40 minutes of access. Compared to males, females overall demonstrated higher ethanol intake and preference across all access weeks, although only half of them drank significantly more than males. Across 24 hours of daily access, both sexes had their highest intake in the first 30 minutes and their lowest in the middle of the light phase of the light/dark cycle. Despite their greater ethanol intake, females did not show significantly different BECs than males. In behavioral tests, females compared to males showed less vertical time in a novel activity chamber, more movement between chambers in a light-dark box, and more nose pokes in a hole-board apparatus. While a history of ethanol drinking led to a trend for reduced vertical time in the activity chamber and increased chamber entries in the light-dark box, this was not sex-dependent. These results suggest that female and male rats could both be tested for acute effects of ethanol after 30 minutes of daily access, but that nuanced considerations should be made in designing these experiments and interpreting the findings.
登录
查看更多内容
影响因子：
2.3
作者：
Loi, Barbara;Colombo, Giancarlo;Gessa, Gian Luigi
通讯作者：
Gessa, Gian Luigi
影响因子：
7.6
作者：
De Oliveira Sergio, Thatiane;Lei, Kelly;Hopf, Frederic W.
通讯作者：
Hopf, Frederic W.
DOI：
10.1016/j.pbb.2015.10.016
发表时间：
2015-12
期刊：
Pharmacology, biochemistry, and behavior
影响因子：
--
作者：
Morales M;McGinnis MM;McCool BA
通讯作者：
McCool BA
DOI：
10.1016/j.pbb.2011.07.017
发表时间：
2011-11
期刊：
Pharmacology, biochemistry, and behavior
影响因子：
--
作者：
Bell RL;Rodd ZA;Smith RJ;Toalston JE;Franklin KM;McBride WJ
通讯作者：
McBride WJ
影响因子：
4.2
作者：
Hasin, Deborah S.;Shmulewitz, Dvora;Keyes, Katherine
通讯作者：
Keyes, Katherine