Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense.
Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense.
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DOI：
10.3389/fmicb.2018.01364
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发表时间：
2018
影响因子：
5.2
通讯作者：
Ji H
中科院分区：
文献类型：
--
作者：
Wang J;Zeng Y;Wang S;Liu H;Zhang D;Zhang W;Wang Y;Ji H
断奶应激使仔猪容易受到病原体感染，从而导致断奶后腹泻，这是一种以严重腹泻和仔猪死亡为特征的严重疾病。产肠毒素大肠杆菌（ETEC）是与断奶后腹泻相关的典型菌株之一。因此，预防和抑制ETEC感染备受关注。益生菌具有抗病原活性，可以抵抗ETEC感染；然而，其根本机制和作用方式尚未阐明。本研究通过不同方法研究了植物乳杆菌ZLP001对ETEC感染的直接和间接保护作用。我们发现，通过牛津杯法，植物乳杆菌 ZLP001 的细菌培养物和培养上清液可以阻止 ETEC 生长，并且在共培养测定中也观察到 ETEC 生长抑制。这种效应被认为主要是由植物乳杆菌 ZLP001 产生的抗菌代谢物引起的。此外，通过显微镜和计数观察植物乳杆菌ZLP001对IPEC-J2细胞的粘附能力。 L. plantarum ZLP001还表现出浓度依赖性的抑制ETEC与IPEC-J2细胞粘附的能力，这主要通过排斥和竞争模式发生。此外，定量实时聚合酶链反应 (qPCR) 分析表明，植物乳杆菌 ZLP001 上调宿主防御肽 (HDP) 的表达，但不会引发炎症反应。此外，植物乳杆菌ZLP001诱导HDP分泌，这增强了与植物乳杆菌ZLP001孵育后IPEC-J2细胞培养上清液的潜在抗菌活性。我们的研究结果表明，植物乳杆菌 ZLP001（一种与仔猪健康相关的肠道乳杆菌）具有抗 ETEC 活性。 L. plantarum ZLP001 可能会阻止 ETEC 生长，抑制 ETEC 对肠粘膜的粘附，并激活分泌抗菌肽的先天免疫反应。 L. plantarum ZLP001 作为一种潜在的益生菌值得研究。
Weaning stress renders piglets susceptible to pathogen infection, which leads to post-weaning diarrhea, a severe condition characterized by heavy diarrhea and mortality in piglets. Enterotoxigenic Escherichia coli (ETEC) is one of typical strains associated with post-weaning diarrhea. Thus, prevention and inhibition of ETEC infection are of great concern. Probiotics possess anti-pathogenic activity and can counteract ETEC infection; however, their underlying mechanisms and modes of action have not yet been clarified. In the present study, the direct and indirect protective effects of Lactobacillus plantarum ZLP001 against ETEC infection were investigated by different methods. We found that bacterial culture and culture supernatant of L. plantarum ZLP001 prevented ETEC growth by the Oxford cup method, and ETEC growth inhibition was observed in a co-culture assay as well. This effect was suggested to be caused mainly by antimicrobial metabolites produced by L. plantarum ZLP001. In addition, adhesion capacity of L. plantarum ZLP001 to IPEC-J2 cells were observed using microscopy and counting. L. plantarum ZLP001 also exhibited a concentration-dependent ability to inhibit ETEC adhesion to IPEC-J2 cells, which mainly occurred via exclusion and competition mode. Furthermore, quantitative real time polymerase chain reaction (qPCR) analysis showed that L. plantarum ZLP001 upregulated the expression of host defense peptides (HDPs) but did not trigger an inflammatory response. In addition, L. plantarum ZLP001 induced HDP secretion, which enhanced the potential antimicrobial activity of IPEC-J2 cell-culture supernatant after incubation with L. plantarum ZLP001. Our findings demonstrate that L. plantarum ZLP001, an intestinal Lactobacillus species associated with piglet health, possesses anti-ETEC activity. L. plantarum ZLP001 might prevent ETEC growth, inhibit ETEC adhesion to the intestinal mucosa, and activate the innate immune response to secret antimicrobial peptides. L. plantarum ZLP001 is worth investigation as a potential probiotics.
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