Quantitative metabolomic profiling of serum, plasma, and urine by (1)H NMR spectroscopy discriminates between patients with inflammatory bowel disease and healthy individuals.
Quantitative metabolomic profiling of serum, plasma, and urine by (1)H NMR spectroscopy discriminates between patients with inflammatory bowel disease and healthy individuals.
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DOI：
10.1021/pr300139q
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发表时间：
2012-06-01
影响因子：
4.4
通讯作者：
Storr M
中科院分区：
文献类型：
--
作者：
Schicho R;Shaykhutdinov R;Ngo J;Nazyrova A;Schneider C;Panaccione R;Kaplan GG;Vogel HJ;Storr M
炎症性肠病（IBD）的血清学生物标志物已经产生了不同的鉴别能力。大量代谢物的定量分析是检测IBD生物标志物的有前途的方法。鉴定患有活动性克罗恩病（CD）和活动性溃疡性结肠炎（UC）的人类受试者，并获得血清、血浆和尿液样本。我们通过使用1H NMR光谱和“靶向分析”来表征44种血清、37种血浆和71种尿液代谢物，以区分患病和非患病个体以及CD和UC队列。我们使用多块主成分分析和分层OPLS-DA来比较来自相同“对象”的几个块（例如，受试者）以检查代谢物的差异。在IBD患者的血清和血浆中，甲醇、甘露糖、甲酸盐、3-甲基-2-氧代戊酸盐和氨基酸（如异亮氨酸）是最显著增加的代谢物，而在尿液中，观察到甘露醇、尿囊素、木糖和肉毒碱的最大增加。UC和CD患者的血清和血浆中尿素和柠檬酸盐均显著降低，而在尿液中观察到甜菜碱和马尿酸盐等降低。血清、血浆和尿液的定量代谢组学分析区分健康和IBD受试者。然而，我们的研究结果表明，CD和UC队列之间的代谢差异不太明显。
Serologic biomarkers for inflammatory bowel disease (IBD) have yielded variable differentiating ability. Quantitative analysis of a large number of metabolites is a promising method to detect IBD biomarkers. Human subjects with active Crohn’s disease (CD) and active ulcerative colitis (UC) were identified, and serum, plasma, and urine specimens were obtained. We characterized 44 serum, 37 plasma, and 71 urine metabolites by use of 1H NMR spectroscopy and “targeted analysis” to differentiate between diseased and non-diseased individuals, as well as between the CD and UC cohorts. We used multiblock principal component analysis and hierarchical OPLS-DA for comparing several blocks derived from the same “objects” (e.g., subject) to examine differences in metabolites. In serum and plasma of IBD patients, methanol, mannose, formate, 3-methyl-2-oxovalerate, and amino acids such as isoleucine were the metabolites most prominently increased, whereas in urine, maximal increases were observed for mannitol, allantoin, xylose, and carnitine. Both serum and plasma of UC and CD patients showed significant decreases in urea and citrate, whereas in urine, decreases were observed, among others, for betaine and hippurate. Quantitative metabolomic profiling of serum, plasma, and urine discriminates between healthy and IBD subjects. However, our results show that the metabolic differences between the CD and UC cohorts are less pronounced.
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影响因子：
56.9
作者：
ERIKSEN, SP
通讯作者：
ERIKSEN, SP
DOI：
10.1016/s0731-7085(03)00410-2
发表时间：
2003-12-04
影响因子：
3.4
作者：
Lenz, EM;Bright, J;Nash, AFP
通讯作者：
Nash, AFP
影响因子：
2.9
作者：
Liu, Linsheng;Aa, Jiye;Wu, Zimei
通讯作者：
Wu, Zimei
影响因子：
4.9
作者：
Hanauer, SB
通讯作者：
Hanauer, SB
影响因子：
2.5
作者：
Balasubramanian, Krithika;Kumar, Sandeep;Jagannathan, Naranamangalam R.
通讯作者：
Jagannathan, Naranamangalam R.