Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization.

Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization.
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钙和信号传感系统对固氮根瘤菌生物膜形成和宿主定殖的影响

DOI:
10.3389/fmicb.2020.563367
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发表时间:
2020
影响因子:
5.2
通讯作者:
Xie Z
Xie Z
中科院分区:
生物学2区
文献类型:
--
作者:
Liu X;Zhang K;Liu Y;Zou D;Wang D;Xie Z

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生物膜的形成对于建立植物-微生物联合体具有重要意义。钙对生物膜形成的作用已在除根瘤菌外的许多细菌中进行了研究。在这项研究中,我们研究了钙离子在Azorhizobium caulindans生物膜形成中的作用,Azorhizobium caulindans在其宿主植物田菁的茎和根中形成根瘤。我们发现,钙是必不可少的A。除了细胞外基质成分、eDNA和蛋白质的存在之外,还可以促进caulindans生物膜的形成。此外,钙介导的生物膜形成进行了测试与趋化性,运动性,环状二GMP合成,和群体感应突变体。最后,发现钙促进S。rostrata根定殖;茎节类总的来说,这些结果表明钙对A. caulindans生物膜的形成,并影响A.茎节线虫和寄主植物。
Biofilm formation is important for establishing plants-microbe associations. The role of calcium on biofilm formation has been studied in many bacteria except rhizobia. In this study, we investigated the role of calcium for biofilm formation in Azorhizobium caulindans, which forms nodules in the stem and root of its host plant Sesbania rostrata. We found that calcium is essential for A. caulindans biofilm formation, in addition to the presence of extracellular matrix components, eDNA and proteins. Also, calcium-mediated biofilm formation was tested with chemotaxis, motility, cyclic di-GMP synthesis, and quorum sensing mutants. Finally, calcium was found to promote S. rostrata root colonization of A. caulinodans. In total, these results show that calcium is essential for A. caulindans biofilm formation, and it affects the interaction between A. caulinodans and host plant.
DOI: 10.1016/j.jmb.2015.06.014
发表时间: 2015-11-20
影响因子: 5.6
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影响因子: 5.1
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