VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine.
VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine.
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VqMYB154促进中国野生葡萄多基因表达并增强对病原体的抵抗力
DOI：
10.1038/s41438-021-00585-0
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发表时间：
2021-07-01
影响因子：
8.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Jiang C;Wang D;Zhang J;Xu Y;Zhang C;Zhang J;Wang X;Wang Y
白藜芦醇在葡萄藤中起着重要的植物抗毒素作用，对人体健康有益。但白藜芦醇积累在增强抗病性中的分子机制尚不清楚。在这里，我们报告的转录因子VqMYB 154从葡萄属quinquangularis登记丹凤2强烈表达人工接种钩虫和调节白藜芦醇积累。与其同源物不同，VqMYB154具有病原体诱导的启动子，并对U. necator，假单胞菌和其他治疗。酵母单杂交和GUS活性测定证实VqMYB 154可以通过直接结合到芪合酶基因VqSTS 9、VqSTS 32和VqSTS 42的启动子来激活芪合酶基因。VqMYB154在葡萄叶片中的过表达导致芪途径的激活、STS基因的上调和芪类化合物的积累。此外，在拟南芥中异源过表达VqMYB154激活了抗性相关基因，并导致更多的细胞程序性死亡和活性氧的积累，从而导致对P. daringae的抗性。这些结果表明，来自毛葡萄丹凤2号的转录因子VqMYB154参与了提高葡萄二苯乙烯类化合物生物合成和积累，增强葡萄藤抗病性的调控机制。
Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accession Danfeng-2 is strongly expressed under artificial inoculation with Uncinula necator and regulates resveratrol accumulation. Unlike its homolog, VqMYB154 has a pathogen-induced promoter and responds to stimulation by U. necator, Pseudomonas syringae, and other treatments. Yeast one-hybrid and GUS activity assays confirmed that VqMYB154 can activate the stilbene synthase genes VqSTS9, VqSTS32, and VqSTS42 by directly binding to their promoters. Overexpression of VqMYB154 in grape leaves resulted in activation of the stilbene pathway, upregulation of STS genes, and accumulation of stilbenoids. In addition, heterologous overexpression of VqMYB154 in Arabidopsis activated resistance-related genes and resulted in greater programmed cell death and accumulation of reactive oxygen species, which led to resistance against P. syringae. These results suggest that the transcription factor VqMYB154 from V. quinquangularis accession Danfeng-2 participates in the regulatory mechanism that improves the biosynthesis and accumulation of stilbenes and enhances resistance to disease in grapevine.
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