Behavioral Effect of Plant Volatiles Binding to Spodoptera littoralis Larval Odorant Receptors.
Behavioral Effect of Plant Volatiles Binding to Spodoptera littoralis Larval Odorant Receptors.
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DOI：
10.3389/fnbeh.2018.00264
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发表时间：
2018
影响因子：
3
通讯作者：
Montagné N
中科院分区：
文献类型：
--
作者：
de Fouchier A;Sun X;Caballero-Vidal G;Travaillard S;Jacquin-Joly E;Montagné N
植食性昆虫利用植物释放的挥发性有机化合物（VOC）来定位它们的宿主。在鳞翅目害虫中，农作物的损害是由幼虫阶段（毛毛虫）造成的，它们广泛以叶子或其他植物组织为食。然而，幼虫寄主植物的选择研究得很少，人们普遍承认，毛虫以雌性产卵的植物为食。毛毛虫的移动性通常被忽视，尽管一些研究表明它们可以定向气味并改变宿主植物。最近，大量的气味受体（ORs）调谐到植物挥发性物质已被鉴定为模式害虫斜纹夜蛾（夜蛾科）。在目前的工作中，我们确定了9个在S.滨毛虫为了了解这些OR是否参与宿主搜索，我们使用幼虫二选择试验测试了它们的配体的行为意义。这种OR引导的方法导致鉴定了9种植物挥发物，即1-己醇、苯甲醇、苯乙酮、苯甲醛、（Z）3-己烯醇、（E）2-己烯醇、吲哚、DMNT和（Z）乙酸3-己烯酯，它们对S.滨毛虫的行为，增加了我们对幼虫嗅觉能力的了解。为了进一步探索OR激活和植物挥发物诱导的行为输出之间的联系，我们使用了建模方法，从而允许识别一些OR的激活与毛虫吸引力有关。这些OR可能是未来植物保护策略的有希望的目标。
Phytophagous insects use volatile organic compounds (VOC) emitted by plants to orient towards their hosts. In lepidopteran pests, crop damages are caused by larval stages—the caterpillars—that feed extensively on leaves or other plant tissues. However, larval host plant choice has been poorly studied, and it is generally admitted that caterpillars feed on the plant where the female laid the eggs. The mobility of caterpillars has been generally overlooked even though several studies showed that they can orient towards odors and change host plant. Recently, a large number of odorant receptors (ORs) tuned to plant volatiles have been characterized in the model pest moth Spodoptera littoralis (Noctuidae). In the present work, we identified nine of these deorphanized ORs as expressed in S. littoralis caterpillars. In order to understand whether these ORs are involved in host searching, we tested the behavioral significance of their ligands using a larval two-choice assay. This OR-guided approach led to the identification of nine plant volatiles, namely 1-hexanol, benzyl alcohol, acetophenone, benzaldehyde, (Z)3-hexenol, (E)2-hexenol, indole, DMNT and (Z)3-hexenyl acetate, which are active on S. littoralis caterpillar behavior, increasing our knowledge on larval olfactory abilities. To further explore the link between OR activation and behavioral output induced by plant volatiles we used a modeling approach, thereby allowing identification of some ORs whose activation is related to caterpillar attraction. These ORs may be promising targets for future plant protection strategies.
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影响因子：
2.3
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
4.6
作者：
Bruce TJA;Aradottir GI;Smart LE;Martin JL;Caulfield JC;Doherty A;Sparks CA;Woodcock CM;Birkett MA;Napier JA;Jones HD;Pickett JA
通讯作者：
Pickett JA