Resonance Scattering Detection of Trace Hg2+ Using Aptamer-modified AuPd Nanoalloy Probe as Catalyst

Resonance Scattering Detection of Trace Hg2+ Using Aptamer-modified AuPd Nanoalloy Probe as Catalyst
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以适配体修饰的AuPd纳米合金探针为催化剂共振散射检测痕量Hg2

DOI:
10.1002/cjoc.201180314
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发表时间:
2011-08
影响因子:
5.4
通讯作者:
Jiang Zhiliang
Jiang Zhiliang
中科院分区:
化学2区
文献类型:
--
作者:
Liu Qingye;Fan Yanyan;Liang Aihui;Jiang Zhiliang

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以柠檬酸钠为稳定剂,NaBH4为还原剂,制备了Au:Pd摩尔比为32:1的5 nm AuPd纳米合金。用适配子修饰AuPd纳米合金,制备了核酸适体-AuPd(AptAuPd)探针,用于检测5.0-1250nmol/L Hg2+的共振散射(RS)。对AptAuPd-Hg2+适体反应溶液进行膜过滤,滤液中的AptAuPd对NiCl2与NaH_2PO_2的缓慢NiP颗粒反应具有很强的催化作用,NiP颗粒在508 nm处有一RS峰。随着Hg2+浓度的增加,RS强度降低。在0.5~1250nmol/L范围内,RS强度与Hg2+浓度呈良好的线性关系,用于实际样品中Hg2+的测定,结果良好。
The 5 nm AuPd nanoalloy in mole ratio of Au:Pd=32:1 was prepared, using sodium citrate as the stabilizing agent and NaBH4 as the reductant. The AuPd nanoalloy was modified by the aptamer to prepare an aptamer- AuPd (AptAuPd) probe for resonance scattering (RS) detection of 5.0–1250 nmol/L Hg2+. The AptAuPd-Hg2+ aptamer reaction solution was filtrated by membrane, and the AptAuPd in the filtrate exhibited strong catalytic effect on the slow NiP particle reaction between NiCl2 and NaH2PO2, and the NiP particles showed a RS peak at 508 nm. The RS intensity decreased when Hg2+ concentration increased. The decreased RS intensity was linear to Hg2+ concentration in the range of 0.5–1250 nmol/L. The RS assays were used to determine Hg2+ in real samples, with good results.
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