Identification and functional analysis of pheromone and receptor genes in the B3 mating locus of Pleurotus eryngii.
Identification and functional analysis of pheromone and receptor genes in the B3 mating locus of Pleurotus eryngii.
复制标题
杏鲍菇B3交配位点信息素和受体基因的鉴定及功能分析
DOI：
10.1371/journal.pone.0104693
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ryu JS
中科院分区：
文献类型：
--
作者：
Kim KH;Kang YM;Im CH;Ali A;Kim SY;Je HJ;Kim MK;Rho HS;Lee HS;Kong WS;Ryu JS
杏鲍菇（Pleurotus eryngii）最近成为一种主要的栽培蘑菇；它利用四极异源性作为其生殖过程的一部分。只有当A和B交配类型相容时，性发育才会进行。这种交配不亲和性有时会限制在位点共享株系内杂交或采用回交策略的育种计划的效率。因此，了解食用菌的交配系统将有助于为新菌株的开发提供一条捷径。本研究分离鉴定了P. eryngii B3位点的信息素和受体基因，并通过转化对这些基因在交配过程中的功能进行了分析。构建基因组DNA文库，绘制整个交配型位点。发现B3位点包含4个信息素前体基因和4个受体基因。值得注意的是，受体PESTE3.3.1在其c端细胞质区只有34个氨基酸残基；因此，它似乎是一种类似受体的基因。实时定量RT-PCR （Real-time qRT-PCR）结果显示，大多数信息素和受体基因在单核细胞中的表达明显高于双核细胞。将信息素基因PEphb3.1、PEphb3.3和受体基因PESTE3.3.1转化为P5 （A3B4）。将这些转化子与一个测试菌株A4B4进行杂交，结果显示其后代具有钳形连接和正常的子实体，这表明了这些基因在交配和结果过程中的作用。这一结果也证实了PESTE3.3.1是一个受体基因。在本研究中，我们鉴定了P. eryngii B3位点的信息素和受体基因，发现其中一些基因似乎在交配和结果过程中起作用。这些结果有助于阐明果实分化机制，提高育种效率。
Pleurotus eryngii has recently become a major cultivated mushroom; it uses tetrapolar heterothallism as a part of its reproductive process. Sexual development progresses only when the A and B mating types are compatible. Such mating incompatibility occasionally limits the efficiency of breeding programs in which crossing within loci-shared strains or backcrossing strategies are employed. Therefore, understanding the mating system in edible mushroom fungi will help provide a short cut in the development of new strains. We isolated and identified pheromone and receptor genes in the B3 locus of P. eryngii and performed a functional analysis of the genes in the mating process by transformation. A genomic DNA library was constructed to map the entire mating-type locus. The B3 locus was found to contain four pheromone precursor genes and four receptor genes. Remarkably, receptor PESTE3.3.1 has just 34 amino acid residues in its C-terminal cytoplasmic region; therefore, it seems likely to be a receptor-like gene. Real-time quantitative RT-PCR (real-time qRT-PCR) revealed that most pheromone and receptor genes showed significantly higher expression in monokaryotic cells than dikaryotic cells. The pheromone genes PEphb3.1 and PEphb3.3 and the receptor gene PESTE3.3.1 were transformed into P5 (A3B4). The transformants were mated with a tester strain (A4B4), and the progeny showed clamp connections and a normal fruiting body, which indicates the proposed role of these genes in mating and fruiting processes. This result also confirms that PESTE3.3.1 is a receptor gene. In this study, we identified pheromone and receptor genes in the B3 locus of P. eryngii and found that some of those genes appear to play a role in the mating and fruiting processes. These results might help elucidate the mechanism of fruiting differentiation and improve breeding efficiency.
登录
查看更多内容
影响因子：
5
作者：
Irie, T;Honda, Y;Kuwahara, M
通讯作者：
Kuwahara, M
DOI：
10.1007/bf00330567
发表时间：
1989-07-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
GIASSON, L;SPECHT, CA;ULLRICH, RC
通讯作者：
ULLRICH, RC
影响因子：
4.4
作者：
Larraya, LM;Pérez, G;Ramírez, L
通讯作者：
Ramírez, L
影响因子：
--
作者：
Kim, H;Borkovich, KA
通讯作者：
Borkovich, KA
影响因子：
3
作者：
Gola, S;Hegner, J;Kothe, E
通讯作者：
Kothe, E