Differential nuclear scaffold/matrix attachment marks expressed genes.
Differential nuclear scaffold/matrix attachment marks expressed genes.
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DOI：
10.1093/hmg/ddn394
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发表时间：
2009-02-15
影响因子：
3.5
通讯作者：
Krawetz SA
中科院分区：
文献类型：
--
作者：
Linnemann AK;Platts AE;Krawetz SA
众所周知，核结构在平衡基因组区域以进行转录中起关键作用。这可以使用建立环结构域的支架/基质附着区（S/MAR）来实现。然而，通过与核支架/基质的连接介导的基因组物理结构的变化与基因表达之间的关系尚不清楚。为了明确S/MARs在组织我们的基因组中的作用，并解决经常相互矛盾的基因座特异性研究，我们通过阵列比较基因组杂交对HeLa S3细胞中人类14-18号染色体上的S/MARs进行了调查。LIS（锂3，5-二碘水杨酸）提取，以确定SAR和2 M NaCl提取，以确定MAR的比较显示，大约一半的网站是共同的。本研究的结果表明，一个基因的SARs 5′与转录本的存在相关，而一个基因中包含的MAR与沉默基因相关。不同的提取方法所揭示的S/MAR的不同功能突出了它们独特的功能贡献。
It is well established that nuclear architecture plays a key role in poising regions of the genome for transcription. This may be achieved using scaffold/matrix attachment regions (S/MARs) that establish loop domains. However, the relationship between changes in the physical structure of the genome as mediated by attachment to the nuclear scaffold/matrix and gene expression is not clearly understood. To define the role of S/MARs in organizing our genome and to resolve the often contradictory loci-specific studies, we have surveyed the S/MARs in HeLa S3 cells on human chromosomes 14–18 by array comparative genomic hybridization. Comparison of LIS (lithium 3,5-diiodosalicylate) extraction to identify SARs and 2 m NaCl extraction to identify MARs revealed that approximately one-half of the sites were in common. The results presented in this study suggest that SARs 5′ of a gene are associated with transcript presence whereas MARs contained within a gene are associated with silenced genes. The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution.
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