Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty.
Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty.
复制标题
DOI：
10.1371/journal.pone.0091592
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Mauney JR
中科院分区：
文献类型：
--
作者：
Chung YG;Tu D;Franck D;Gil ES;Algarrahi K;Adam RM;Kaplan DL;Estrada CR Jr;Mauney JR
在兔尿道修复模型中，研究了来源于桑蚕丝素蛋白的脱细胞支架支持功能性组织再生的能力。采用溶剂流延/盐浸法结合丝素蛋白膜流延制备了双层丝素蛋白基质，以生成由均匀丝素蛋白膜支撑的多孔泡沫。将丝素蛋白移植物（组1，N = 4）（宽×长，1×2 cm 2）植入成年雄性家兔体内，进行尿道覆盖成形术，植入3 m。 平行对照组由接受小肠粘膜下层（SIS）植入物（第2组，N = 4）或单独尿道切开术（第3组，N = 3）的动物组成。 所有组中的动物在计划安乐死前均表现出100%的存活率，并在初始导管插入7天后实现自愿排尿。术后3个月时，每个植入物组的逆行尿道造影显示尿道口径较宽，器官连续性得到保持，与术前和尿道切开术对照相似，没有造影剂外渗、狭窄、瘘管或结石形成的证据。组织学（苏木精-伊红和Masson三色）、免疫组织化学和组织形态学分析表明，丝素蛋白和SIS支架分别促进了原始缺损部位的平滑肌和上皮组织再生的相似程度，并分别具有显著的收缩蛋白（α-平滑肌肌动蛋白和SM 22 α）和细胞角蛋白表达。新生神经支配和血管化也明显的突触素阳性神经元细胞和血管内衬与CD 31表达内皮细胞的所有再生组织。术后3个月，丝素蛋白支架引起了最小的急性炎症反应，其特征在于存在嗜酸性粒细胞，而SIS基质促进了单核细胞浸润动员所指示的慢性炎症反应。本研究的结果表明，双层丝素蛋白支架代表了用于覆盖尿道成形术的有前途的生物材料，与传统的SIS支架相比，能够促进类似程度的组织再生，但具有降低的免疫原性。
Acellular scaffolds derived from Bombyx mori silk fibroin were investigated for their ability to support functional tissue regeneration in a rabbit model of urethra repair. A bi-layer silk fibroin matrix was fabricated by a solvent-casting/salt leaching process in combination with silk fibroin film casting to generate porous foams buttressed by homogeneous silk fibroin films. Ventral onlay urethroplasty was performed with silk fibroin grafts (Group 1, N = 4) (Width×Length, 1×2 cm2) in adult male rabbits for 3 m of implantation. Parallel control groups consisted of animals receiving small intestinal submucosa (SIS) implants (Group 2, N = 4) or urethrotomy alone (Group 3, N = 3). Animals in all groups exhibited 100% survival prior to scheduled euthanasia and achieved voluntary voiding following 7 d of initial catheterization. Retrograde urethrography of each implant group at 3 m post-op revealed wide urethral calibers and preservation of organ continuity similar to pre-operative and urethrotomy controls with no evidence of contrast extravasation, strictures, fistulas, or stone formation. Histological (hematoxylin and eosin and Masson's trichrome), immunohistochemical, and histomorphometric analyses demonstrated that both silk fibroin and SIS scaffolds promoted similar extents of smooth muscle and epithelial tissue regeneration throughout the original defect sites with prominent contractile protein (α-smooth muscle actin and SM22α) and cytokeratin expression, respectively. De novo innervation and vascularization were also evident in all regenerated tissues indicated by synaptophysin-positive neuronal cells and vessels lined with CD31 expressing endothelial cells. Following 3 m post-op, minimal acute inflammatory reactions were elicited by silk fibroin scaffolds characterized by the presence of eosinophil granulocytes while SIS matrices promoted chronic inflammatory responses indicated by mobilization of mononuclear cell infiltrates. The results of this study demonstrate that bi-layer silk fibroin scaffolds represent promising biomaterials for onlay urethroplasty, capable of promoting similar degrees of tissue regeneration in comparison to conventional SIS scaffolds, but with reduced immunogenicity.
登录
查看更多内容
影响因子：
6.6
作者：
Atala, A;Guzman, L;Retik, AB
通讯作者：
Retik, AB
影响因子：
3.7
作者：
Elgammal, Mohammed Abd-Alla
通讯作者：
Elgammal, Mohammed Abd-Alla
影响因子：
2.1
作者：
Kropp, BP;Ludlow, JK;Thor, KB
通讯作者：
Thor, KB
影响因子：
18.6
作者：
Mawhinney, Michael;Mariotti, Angelo
通讯作者：
Mariotti, Angelo
影响因子：
--
作者：
Ceonzo, K;Gaynor, A;Stahl, GL
通讯作者：
Stahl, GL