Rap1 maintains adhesion between cells to affect Egfr signaling and planar cell polarity in Drosophila.
Rap1 maintains adhesion between cells to affect Egfr signaling and planar cell polarity in Drosophila.
复制标题
DOI：
10.1016/j.ydbio.2009.06.032
复制
发表时间：
2009-09-01
影响因子：
2.7
通讯作者：
Curtiss, Jennifer
中科院分区：
文献类型：
--
作者：
O'Keefe, David D.;Gonzalez-Nino, Eduardo;Burnett, Micheal;Dylla, Layne;Lambeth, Stacey M.;Licon, Elizabeth;Amesoli, Cassandra;Edgar, Bruce A.;Curtiss, Jennifer
小的GTPase Rap1在许多发育背景下影响细胞粘附和细胞运动。果蝇翅膀上皮Rap1的缺失会破坏粘附连接的定位，导致突变细胞分散，并显著改变上皮细胞的形状。虽然Rap1失活的粘附后果在该系统中已经得到了很好的描述，但对细胞信号传导、细胞命运规范和组织分化的影响尚不清楚。在这里，我们证明了依赖egfr的细胞类型从Rap1突变组织中丢失，这是de -钙粘蛋白错误定位的间接后果。发育中的翅膀和眼睛中缺乏Rap1的细胞能够响应Egfr信号，这表明Egfr/Ras/MAPK信号转导不需要Rap1。相反，Rap1调节维持细胞间Egfr信号和翼静脉和光感受器分化所必需的粘附接触。Rap1对于这些组织中的平面细胞极性也是必需的。在Rap1突变体组织中，翼毛排列和眼毛旋转（分别是翅膀和眼睛中平面细胞极性的功能读数）都受到影响。最后，我们发现Rap1通过效应物Canoe来调节这些发育过程。
The small GTPase Rap1 affects cell adhesion and cell motility in numerous developmental contexts. Loss of Rap1 in the Drosophila wing epithelium disrupts adherens junction localization, causing mutant cells to disperse, and dramatically alters epithelial cell shape. While the adhesive consequences of Rap1 inactivation have been well described in this system, the effects on cell signaling, cell fate specification, and tissue differentiation are not known. Here we demonstrate that Egfr-dependent cell types are lost from Rap1 mutant tissue as an indirect consequence of DE-cadherin mis-localization. Cells lacking Rap1 in the developing wing and eye are capable of responding to an Egfr signal, indicating that Rap1 is not required for Egfr/Ras/MAPK signal transduction. Instead, Rap1 regulates adhesive contacts necessary for maintenance of Egfr signaling between cells, and differentiation of wing veins and photoreceptors. Rap1 is also necessary for planar cell polarity in these tissues. Wing hair alignment and ommatidial rotation, functional readouts of planar cell polarity in the wing and eye respectively, are both affected in Rap1 mutant tissue. Finally, we show that Rap1 acts through the effector Canoe to regulate these developmental processes.
登录
查看更多内容
影响因子：
9.2
作者：
Baonza, A;Casci, T;Freeman, M
通讯作者：
Freeman, M
影响因子：
64.8
作者：
BRUNNER, D;DUCKER, K;KLAMBT, C
通讯作者：
KLAMBT, C
影响因子：
11.4
作者：
Frische, Ester W.;Pellis-van Berkel, Wendy;Zwartkruis, Fried J. T.
通讯作者：
Zwartkruis, Fried J. T.
影响因子：
4.6
作者：
Gaengel, K;Mlodzik, M
通讯作者：
Mlodzik, M
影响因子：
64.5
作者：
Freeman, M
通讯作者：
Freeman, M