First Report of the Plasmid-mediated fosB Gene in Enterococcus faecalis from Pigs.
First Report of the Plasmid-mediated fosB Gene in Enterococcus faecalis from Pigs.
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DOI：
10.3390/genes12111684
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发表时间：
2021-10-23
期刊：
影响因子：
3.5
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Wang X;Gao Y;Liu X;Sun N;Huang J;Wang L
由于磷霉素耐药的传播增加和临床治疗选择有限，质粒介导的磷霉素决定簇是全球公共卫生问题。肠球菌属磷霉素耐药性及分子遗传学研究。仍然缺乏。在这项研究中，我们在猪粪肠球菌和所有磷霉素耐药肠球菌中发现了第一个质粒介导的fosB。(FRE)分离株具有多重耐药性。S1-PFGE、Southern杂交和接合试验表明，fosB基因定位在约54.7kb的可转移质粒上。相对竞争实验证实，携带fosB的质粒在受体大肠杆菌中的适应性受损。粪生菌JH 2 -2。Illumina和MinION测序数据显示，E.粪肠球菌ES-1和ES-2菌株属于新型ST（ST 964），有71个SNPs差异。WGS结果显示，不同种属间fosB的遗传环境存在差异，携带fosB的菌株中存在利奈唑胺耐药基因optrA。综上所述，我们首次报道了质粒介导的fosB在大肠杆菌中的表达。猪的粪便而且，fosB和optrA的共存对公共健康构成严重威胁。
Plasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among Enterococcus spp. is still lacking. In this study, we found the first plasmid-medieted fosB in Enterococcus faecalis from pigs, and all the fosfomycin resistant Enterococcus spp. (FRE) isolates were multi-drug resistant. S1-PFGE, Southern blot and conjugation experiments indicated that the fosB gene located on ~54.7 kb transferable plasmids. Relative competition assay confirmed that the fosB-carrying plasmid impaired fitness in recipient E. faecalis JH2-2. Illumina and the MinION sequencing data revealed that both E. faecalis ES-1 and ES-2 isolates belonged to novel ST (ST964), and had 71 SNPs difference. WGS showed that the genetic environments of fosB were diverse among different species, and the linezolid resistance gene optrA was found in the fosB-carrying strains. To summarize, for the first time, we reported plasmid-mediated fosB in E. faecalis from pigs. And, the co-occurrence of fosB and optrA pose a serious threat to public health.
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影响因子：
5.4
作者：
Silver, Lynn L.
通讯作者：
Silver, Lynn L.
DOI：
10.1111/j.1749-6632.1974.tb43277.x
发表时间：
1974-01-01
影响因子：
5.2
作者：
KAHAN, FM;KAHAN, JS;KROPP, H
通讯作者：
KROPP, H
影响因子：
3.3
作者：
Leiro, J;Toranzo, AE;Ubeira, FM
通讯作者：
Ubeira, FM
影响因子：
5.2
作者：
SNELL, JJS;BROWN, DFJ;GEORGE, R
通讯作者：
GEORGE, R
影响因子：
3.3
作者：
Yao, Hong;Wu, Dongfang;Liao, Kang
通讯作者：
Liao, Kang